Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605155219_21ry AGGAGCATCCTACCTCCCATAGTCCATAATTG(+CAGTGT)GTACCCAGCAAGGCTTTCTTCTCTAGAATAG NGG 10 8 17 8 24 0 100 5 10
    240605155217_qrwh GGCTACATGCACGGGCTTCTCTGAGATTCCAG(+CAGTGT)GTACCAGTTCCTTCCGGGTTATGTAATATTA NGG 10 8 17 8 24 0 100 5 10
    240605155216_9h08 GTCTCTAATATTACATAACCCGGAAGGAACTG(+CAGTGT)GTACCTGGAATCTCAGAGAAGCCCGTGCATG NGG 10 8 17 8 24 0 100 5 10
    240605155215_pdb3 CACACCGGAAGTGAGAGGTGTCTCAGTGGTAG(+CAGTGT)GTACCAATCTCCAACCAAGACCCAGGCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605155213_j9xt TTCAGGGAAGCCTGGGTCTTGGTTGGAGATTG(+CAGTGT)GTACCTACCACTGAGACACCTCTCACTTCCG NGG 10 8 17 8 24 0 100 5 10
    240605155210_jdu3 GCAGGCAGCAAGGAGACCTGAGGTGGCCCTGG(+CAGTGT)GTACCAGGACCCCAGAGTAGAGGTGAGGATG NGG 10 8 17 8 24 0 100 5 10
    240605155208_ajlp CTGTGCATCCTCACCTCTACTCTGGGGTCCTG(+CAGTGT)GTACCCAGGGCCACCTCAGGTCTCCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605155207_6nlm CCACCAAGTGCACAGCACCCACCCCGTATGTG(+CAGTGT)GTACCCTGTACGCGTGGATCTTTGTGTGCAC NGG 10 8 17 8 24 0 100 5 10
    240605155205_3xc8 GATCTGTGCACACAAAGATCCACGCGTACAGG(+CAGTGT)GTACCACATACGGGGTGGGTGCTGTGCACTT NGG 10 8 17 8 24 0 100 5 10
    240605155203_pe4t CCTCTGGGACGTCTGTGATGCTCCTTCTCTGG(+CAGTGT)GTACCTGAAGAGGCTCCTCCACGACCAAGCC NGG 10 8 17 8 24 0 100 5 10