Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605155252_rn4e AGCAGTGGAAATCATAGTCCTTGGCTGGCATG(+CAGTGT)GATCCAACCCCAGGCTTGTGATTGGGTGGTG NGG 10 8 17 8 24 0 100 5 10
    240605155250_sant GCTTGGCTACCTGTAGACTCTAATCCTAAGTG(+CAGTGT)GATCCAGAATTGCTTTCCCAAGTCTAACTTC NGG 10 8 17 8 24 0 100 5 10
    240605155249_cans GAACTGAAGTTAGACTTGGGAAAGCAATTCTG(+CAGTGT)GATCCACTTAGGATTAGAGTCTACAGGTAGC NGG 10 8 17 8 24 0 100 5 10
    240605155248_jetv AACCTAGAAACTGTAGGTGGGCATATATCAGG(+CAGTGT)GTACCTATGCCACCGCCAACTTCTCCTTTGT NGG 10 8 17 8 24 0 100 5 10
    240605155246_d4ir CTTGGACAAAGGAGAAGTTGGCGGTGGCATAG(+CAGTGT)GTACCCTGATATATGCCCACCTACAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605155244_65bz AATGTACTAAATGATTCTCCTCCTCTCTCCTG(+CAGTGT)GTACCTGGAGACCCCTGGCTATCATGGGATT NGG 10 8 17 8 24 0 100 5 10
    240605155242_ovnw TCAGTAATCCCATGATAGCCAGGGGTCTCCAG(+CAGTGT)GTACCAGGAGAGAGGAGGAGAATCATTTAGT NGG 10 8 17 8 24 0 100 5 10
    240605155241_jhdq ACTCAGAAAAGAACCATAGGCACACACGTGTG(+CAGTGT)GATCCTATATCTTGATATTATATAGATACAC NGG 10 8 17 8 24 0 100 5 10
    240605155240_5bbo GGATGGTGTATCTATATAATATCAAGATATAG(+CAGTGT)GATCCACACGTGTGTGCCTATGGTTCTTTTC NGG 10 8 17 8 24 0 100 5 10
    240605155238_b279 GGCTATTCCTTGTCCCTACACTGCTCTCTTGG(+CAGTGT)GTACCTCTGGGGCACCCTGGCAGGAATGAGA NGG 10 8 17 8 24 0 100 5 10