Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605155352_jiy5 AACACTCATCGTCCTGGACATGTGGGGGGCAG(+CAGTGT)GATCCTGGTTTATGTTTTACACTTCTAAATA NGG 10 8 17 8 24 0 100 5 10
    240605155351_wpxm TAAATTTCACCCTCTGCATTACTCACAGAGTG(+CAGTGT)GATCCATTGGAGTTTCAGCTGTATGCTAGTT NGG 10 8 17 8 24 0 100 5 10
    240605155349_nxf1 AGCTGAACTAGCATACAGCTGAAACTCCAATG(+CAGTGT)GATCCACTCTGTGAGTAATGCAGAGGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605155347_6yez GCCAACATTCCTAAGACTGAACCAACATCCAG(+CAGTGT)GTACCCATTTTCTCAGGTTTCCCAGAGATAT NGG 10 8 17 8 24 0 100 5 10
    240605155345_75du GCCACATATCTCTGGGAAACCTGAGAAAATGG(+CAGTGT)GTACCTGGATGTTGGTTCAGTCTTAGGAATG NGG 10 8 17 8 24 0 100 5 10
    240605155344_r941 AGAGAAGAACTCTTATAGACTTGGGGAGAGAG(+CAGTGT)GTACCAACCTTGACATTGACTGTACTGGTGA NGG 10 8 17 8 24 0 100 5 10
    240605155343_00i6 AATACTCACCAGTACAGTCAATGTCAAGGTTG(+CAGTGT)GTACCTCTCTCCCCAAGTCTATAAGAGTTCT NGG 10 8 17 8 24 0 100 5 10
    240605155341_yc9z TGGAACCCAGTTACAGAAATTGTGTGATATAG(+CAGTGT)GTACCTTTTTCTCTTTCTCTGTCCAAGACAT NGG 10 8 17 8 24 0 100 5 10
    240605155340_tp0f AAAGCATGTCTTGGACAGAGAAAGAGAAAAAG(+CAGTGT)GTACCTATATCACACAATTTCTGTAACTGGG NGG 10 8 17 8 24 0 100 5 10
    240605155339_mki5 CCATTTCAAGGTGCAGTGTGAAGTATGACTAG(+CAGTGT)GTACCTCTCCATTTATTGAGGCTGGAACAGG NGG 10 8 17 8 24 0 100 5 10