Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605155338_vfty GATTTCCTGTTCCAGCCTCAATAAATGGAGAG(+CAGTGT)GTACCTAGTCATACTTCACACTGCACCTTGA NGG 10 8 17 8 24 0 100 5 10
    240605155336_fi3u CTTCAGCTGGAGTGTGCTCTGCTGGTGCAGTG(+CAGTGT)GATCCTAGTGTGGTTCCCAGTACTCAACCTG NGG 10 8 17 8 24 0 100 5 10
    240605155335_w0sm CCAACCAGGTTGAGTACTGGGAACCACACTAG(+CAGTGT)GATCCACTGCACCAGCAGAGCACACTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605155334_wsoz AGAGACAAAGGACACAGGAACCCGGCTGACTG(+CAGTGT)GTACCAAAGTGCACTCTCTTCAGGTGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605155333_8953 TGTGTAGAGGCACCTGAAGAGAGTGCACTTTG(+CAGTGT)GTACCAGTCAGCCGGGTTCCTGTGTCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605155332_cbex TGTCTTTATTCTAATGTCTTTGGTGTGGGAAG(+CAGTGT)GTACCTCATAGGCTGCCAAAAGAGAAATGCA NGG 10 8 17 8 24 0 100 5 10
    240605155330_lxxf ATGTTTGCATTTCTCTTTTGGCAGCCTATGAG(+CAGTGT)GTACCTTCCCACACCAAAGACATTAGAATAA NGG 10 8 17 8 24 0 100 5 10
    240605155329_hqgj TGGGTATGGATGGAGCCACACTCCTATGCAAG(+CAGTGT)GATCCATTTCCATGCAAGATTGTCAATTCTT NGG 10 8 17 8 24 0 100 5 10
    240605155328_7wt7 TCTGGAAGAATTGACAATCTTGCATGGAAATG(+CAGTGT)GATCCTTGCATAGGAGTGTGGCTCCATCCAT NGG 10 8 17 8 24 0 100 5 10
    240605155327_vxnw TTTTTAAGATGCTTCTGGAGATTTAGAATCAG(+CAGTGT)GATCCAAAATAAGGCTGGGATTCAGCATCCA NGG 10 8 17 8 24 0 100 5 10