Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605155145_fc3n GGTATTCTACCCTTGAGATACATCCCAGCTAG(+CAGTGT)GATCCCCTTGAACCAGTGACTCTGCGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240605155144_c397 AACACGGAACTCCCAGGATTCTGGAATGACAG(+CAGTGT)GATCCTCCCAGAAGGTGTGTATACCCCTTAA NGG 10 8 17 8 24 0 100 5 10
    240605155142_3h4n TGTGTTTAAGGGGTATACACACCTTCTGGGAG(+CAGTGT)GATCCTGTCATTCCAGAATCCTGGGAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240605155141_oxml GGGAAAGTCACATGCTAGCCTCTCCAGCTTCG(+CAGTGT)GATCCAGGGCTCCCTTTGATGTGACAACCAT NGG 10 8 17 8 24 0 100 5 10
    240605155138_bild TACCAATGGTTGTCACATCAAAGGGAGCCCTG(+CAGTGT)GATCCGAAGCTGGAGAGGCTAGCATGTGACT NGG 10 8 17 8 24 0 100 5 10
    240605155136_bc9y GTGCCCGGTGTGCAGGCACCAAGGAGACACAG(+CAGTGT)GTACCAGCAGACCACCACTGGTGACATCATC NGG 10 8 17 8 24 0 100 5 10
    240605155135_eu07 CTGGTGATGATGTCACCAGTGGTGGTCTGCTG(+CAGTGT)GTACCTGTGTCTCCTTGGTGCCTGCACACCG NGG 10 8 17 8 24 0 100 5 10
    240605155133_xqzx AGTCCTCACCGAAGTCACATCAACTTCCTATG(+CAGTGT)GTACCTCTCATGAACCTGGGGTGCTTCTTAC NGG 10 8 17 8 24 0 100 5 10
    240605155132_dyvw ACGGGGTAAGAAGCACCCCAGGTTCATGAGAG(+CAGTGT)GTACCATAGGAAGTTGATGTGACTTCGGTGA NGG 10 8 17 8 24 0 100 5 10
    240605155131_6obo AGTGCTTGTCTTACATGCACAGGATCTGGTTG(+CAGTGT)GATCCCCATGCATCATTGACACTGGGTATTG NGG 10 8 17 8 24 0 100 5 10