Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605155055_w2ti TCTCCAGTCTGGGCACAGCCGGAACTGACCGG(+CAGTGT)GATCCAGAAATGTCACAGATGCGCAAGCCGC NGG 10 8 17 8 24 0 100 5 10
    240605155053_rvd6 GTGGTTGCAACTTCTGGAGCAGGTGGGTGTGG(+CAGTGT)GATCCCAGGTGTAGTGTTGGCTGAGCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240605155050_83np TGTCATCTGAGCTCAGCCAACACTACACCTGG(+CAGTGT)GATCCCACACCCACCTGCTCCAGAAGTTGCA NGG 10 8 17 8 24 0 100 5 10
    240605155049_1w6o AGAGTCCCAACTCCAGTGAGCAGTAGGGCAGG(+CAGTGT)GATCCCACTCAGATCACAAAGTCATCATGGT NGG 10 8 17 8 24 0 100 5 10
    240605155047_0uk3 CGCGGACCATGATGACTTTGTGATCTGAGTGG(+CAGTGT)GATCCCTGCCCTACTGCTCACTGGAGTTGGG NGG 10 8 17 8 24 0 100 5 10
    240605155045_gtaf TGGCAGTCATAGTGCCTAGAGCTTCTTCCCTG(+CAGTGT)GTACCCAGGAGTTCCTCACCACTCCCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605155042_n1fj TAACTTCCAGGGGAGTGGTGAGGAACTCCTGG(+CAGTGT)GTACCAGGGAAGAAGCTCTAGGCACTATGAC NGG 10 8 17 8 24 0 100 5 10
    240605155041_2vsk AGCTGTCTGCCCTTCATGCTCCAGGGCAGCTG(+CAGTGT)GTACCGTGGAAAGTGCCTGGGGTGGTTTTGC NGG 10 8 17 8 24 0 100 5 10
    240605155039_2znq TATTTGCAAAACCACCCCAGGCACTTTCCACG(+CAGTGT)GTACCAGCTGCCCTGGAGCATGAAGGGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605155038_xx77 TACAGAGTAAGAGCCTGTCTCAAAAAAAAAAG(+CAGTGT)GTACCATTTTTATTGTGAGGGCTCCATGTAG NGG 10 8 17 8 24 0 100 5 10