Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154949_k5bw GCCAGAAGAGTCTTCCCAGGTGCCTGGCCAGG(+CAGTGT)GTACCTGGTGAAAGGTGGCCTTGCCTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240605154947_rv9x TCCTGGGCAGCCTGGAGGGCTGGCATGCCTGG(+CAGTGT)GATCCTCGGCCACCCAGGATCTTGGAGACAA NGG 10 8 17 8 24 0 100 5 10
    240605154945_mn19 GACACTTGTCTCCAAGATCCTGGGTGGCCGAG(+CAGTGT)GATCCCAGGCATGCCAGCCCTCCAGGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605154943_3dly GCCAGGACAGTCTTCTTCAGCTGGGCAGGCGG(+CAGTGT)GATCCTGGGAAAGCGAAGTGGCAGAAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605154940_4kd1 CAATGCCAGCTTCTGCCACTTCGCTTTCCCAG(+CAGTGT)GATCCCGCCTGCCCAGCTGAAGAAGACTGTC NGG 10 8 17 8 24 0 100 5 10
    240605154939_8wll TCACACAATAAGATTAAGTTTTAGAAACTAAG(+CAGTGT)GATCCCACCTTTTTTTCTTGTTTAAAATAAT NGG 10 8 17 8 24 0 100 5 10
    240605154937_feuy AATAAATTATTTTAAACAAGAAAAAAAGGTGG(+CAGTGT)GATCCTTAGTTTCTAAAACTTAATCTTATTG NGG 10 8 17 8 24 0 100 5 10
    240605154936_8a28 AGGATACCATGGTCTCCAGGGGCTAAGAGAAG(+CAGTGT)GTACCTCTCTCAAGGGAAGGAAAACACAGTC NGG 10 8 17 8 24 0 100 5 10
    240605154935_bwqi TTTAGGACTGTGTTTTCCTTCCCTTGAGAGAG(+CAGTGT)GTACCTTCTCTTAGCCCCTGGAGACCATGGT NGG 10 8 17 8 24 0 100 5 10
    240605154933_extx TCCTTTGGGGGCAACAGAGTGGCTGACAAAGG(+CAGTGT)GTACCGTGAGAATCCCATTCTGAGGACAGGG NGG 10 8 17 8 24 0 100 5 10