Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154845_cq24 TCAAGAAGCCAGCGCAAGAGATTTCAGCCTGG(+CAGTGT)GTACCAGCAACTTCCAGTTCTAAGTCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605154844_m2a3 TGTGGATAACATATTATGTATATATGTAATGG(+CAGTGT)GTACCAGGCCAGGTTTATATTTTTTTCTTTA NGG 10 8 17 8 24 0 100 5 10
    240605154842_63wf ACGTCTAAAGAAAAAAATATAAACCTGGCCTG(+CAGTGT)GTACCCATTACATATATACATAATATGTTAT NGG 10 8 17 8 24 0 100 5 10
    240605154840_237b GCTCACAACCATGCATAACTCAAGTTTTAAGG(+CAGTGT)GATCCAGTGCTGACTTCTGGCCTCTGAGGGT NGG 10 8 17 8 24 0 100 5 10
    240605154839_umh3 GCAGTACCCTCAGAGGCCAGAAGTCAGCACTG(+CAGTGT)GATCCCTTAAAACTTGAGTTATGCATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605154837_nbi8 TTGGGGGAGATGCAGCACACACCCCAGATAGG(+CAGTGT)GATCCTATGACTGACCAACAAAAGTACAGGT NGG 10 8 17 8 24 0 100 5 10
    240605154835_85a1 GTGGTACCTGTACTTTTGTTGGTCAGTCATAG(+CAGTGT)GATCCCTATCTGGGGTGTGTGCTGCATCTCC NGG 10 8 17 8 24 0 100 5 10
    240605154834_yk1l CATGCCATTTTAGGGCCATTGTGATTCTCAGG(+CAGTGT)GATCCTAGGTAAAAATCCTGAATCTTTTGGG NGG 10 8 17 8 24 0 100 5 10
    240605154832_1j7a TCTTACCCAAAAGATTCAGGATTTTTACCTAG(+CAGTGT)GATCCCTGAGAATCACAATGGCCCTAAAATG NGG 10 8 17 8 24 0 100 5 10
    240605154830_3zh4 TCTTGCTTGTCAACTTCAGAGATAGCTGGAAG(+CAGTGT)GATCCTGAGACTGTATGGCAGGAGAGTTGAG NGG 10 8 17 8 24 0 100 5 10