Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154901_1q7o AATGCATTGTTTCAACAAGTTGGACTCTGGTG(+CAGTGT)GTACCTGTACTTTTGTTGGTCAGTCATAGGA NGG 10 8 17 8 24 0 100 5 10
    240605154900_ima1 TAATTTCTCATTACCCTCTGTAAACACAATGG(+CAGTGT)GTACCCTTTGTTTTCCTCTCCTAAAAATAAC NGG 10 8 17 8 24 0 100 5 10
    240605154858_hgwa ACAAAGTTATTTTTAGGAGAGGAAAACAAAGG(+CAGTGT)GTACCCATTGTGTTTACAGAGGGTAATGAGA NGG 10 8 17 8 24 0 100 5 10
    240605154857_6bc7 AATGTTGGGAGATCCCAGAAGCTATAGTGAAG(+CAGTGT)GTACCTGAAGGGCATTGCTAGTAGGATAGAG NGG 10 8 17 8 24 0 100 5 10
    240605154855_8mmx TCCCACTCTATCCTACTAGCAATGCCCTTCAG(+CAGTGT)GTACCTTCACTATAGCTTCTGGGATCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240605154854_471f CTACAGGGAGGGAATCAGGCCACACTGCTGAG(+CAGTGT)GTACCCTGTGGTTCCTTTATAAGCTTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240605154852_cuo9 ACCAAACAAGAAGCTTATAAAGGAACCACAGG(+CAGTGT)GTACCTCAGCAGTGTGGCCTGATTCCCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605154851_thnt TGACCTCAGCAAATCCTCCTTTTAACTCATGG(+CAGTGT)GTACCCAGACTCGAACTATTTCACAATTCAC NGG 10 8 17 8 24 0 100 5 10
    240605154849_pn8y TGGTTGTGAATTGTGAAATAGTTCGAGTCTGG(+CAGTGT)GTACCCATGAGTTAAAAGGAGGATTTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605154847_orqy AAACTTGGCTGACTTAGAACTGGAAGTTGCTG(+CAGTGT)GTACCCAGGCTGAAATCTCTTGCGCTGGCTT NGG 10 8 17 8 24 0 100 5 10