Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154814_t9dn TGAACTATATTAGCAGCCCAGCAAGCCTCAGG(+CAGTGT)GATCCTCTTGTCTCCACCTCCCAACGAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605154813_81n3 TCTACACCGAGTTACTGATAAACCTCATCAAG(+CAGTGT)GTACCTGTTCTGTCAGGTGGTGTCCTAATCT NGG 10 8 17 8 24 0 100 5 10
    240605154811_hr0d AGAGCAGATTAGGACACCACCTGACAGAACAG(+CAGTGT)GTACCTTGATGAGGTTTATCAGTAACTCGGT NGG 10 8 17 8 24 0 100 5 10
    240605154810_rfve TGTCCACACATCCCTCTCTAAGCCCAGGATAG(+CAGTGT)GTACCAATATAGGTAACTCTGCATTTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605154809_x6bm CTTATGGGAAAAATGCAGAGTTACCTATATTG(+CAGTGT)GTACCTATCCTGGGCTTAGAGAGGGATGTGT NGG 10 8 17 8 24 0 100 5 10
    240605154808_fh7l TACACCTCTAGGAACTGAGGCAGCTTCAGAAG(+CAGTGT)GTACCTGCCAGATGTTGCTGTGGGAAGCATG NGG 10 8 17 8 24 0 100 5 10
    240605154806_3rqz AAGGCCATGCTTCCCACAGCAACATCTGGCAG(+CAGTGT)GTACCTTCTGAAGCTGCCTCAGTTCCTAGAG NGG 10 8 17 8 24 0 100 5 10
    240605154803_hlet GATGGCTTACAACTGTAACCCTGGTTCCAGGG(+CAGTGT)GATCCAGTACCTTCTGACCTGCTCGGGTGCC NGG 10 8 17 8 24 0 100 5 10
    240605154802_aqzj TTCCTGGCACCCGAGCAGGTCAGAAGGTACTG(+CAGTGT)GATCCCCTGGAACCAGGGTTACAGTTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240605154800_ab4f TTCCTTGAAGTGAGGTCAAGGTCATGTGGCCG(+CAGTGT)GATCCTATACTCACCACTGGCTGTCTTTCTC NGG 10 8 17 8 24 0 100 5 10