Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154743_eu38 CTAACCACTGCCCTCCTGGAACTGCTGTGCTG(+CAGTGT)GATCCAAAGGCCTCCTCACCAGGAAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605154740_d12a GGGCCAGAGCCTTCCTGGTGAGGAGGCCTTTG(+CAGTGT)GATCCAGCACAGCAGTTCCAGGAGGGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605154738_37wz ATAGGTGCTGGAGAAGCGGGGACCGCAGTGGG(+CAGTGT)GTACCATCAGGCTCGGGGTAAGGTAGAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605154737_4x0k TTACCCTTTTCTACCTTACCCCGAGCCTGATG(+CAGTGT)GTACCCCACTGCGGTCCCCGCTTCTCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605154735_11d1 CTCCTCTACACAGCAGCAACTAAAATGATCAG(+CAGTGT)GATCCTTCACTGGAGCCCTAGAGACTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240605154734_a4ny ATCTTCTGGGAGTCTCTAGGGCTCCAGTGAAG(+CAGTGT)GATCCTGATCATTTTAGTTGCTGCTGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240605154733_2q2r CATACCTAGGAGCAAGCACTGATCGCTGAGCG(+CAGTGT)GTACCAAGAGGAGCCTCTCCTTTTACTGGAT NGG 10 8 17 8 24 0 100 5 10
    240605154732_flkf TCTCCATCCAGTAAAAGGAGAGGCTCCTCTTG(+CAGTGT)GTACCGCTCAGCGATCAGTGCTTGCTCCTAG NGG 10 8 17 8 24 0 100 5 10
    240605154730_bsx1 GGCCCCATCATTTGTAGATGTAGATCTTGTAG(+CAGTGT)GTACCTGCTATGGCAACTCATTTTATTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605154729_c0m2 GTACTTGAGAATAAAATGAGTTGCCATAGCAG(+CAGTGT)GTACCTACAAGATCTACATCTACAAATGATG NGG 10 8 17 8 24 0 100 5 10