Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154637_wlex GCCTTGTCTAGGAACACAGAAAGGAGATGATG(+CAGTGT)GTACCTACTGGCCTTGGACTCTTCTGCGTGA NGG 10 8 17 8 24 0 100 5 10
    240605154636_hifg GTTTGTCACGCAGAAGAGTCCAAGGCCAGTAG(+CAGTGT)GTACCATCATCTCCTTTCTGTGTTCCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240605154634_ne6o TGTAAAATTCCAGTGACTCAAATGGCTTTAGG(+CAGTGT)GATCCCCTGGAGACGGTTCAGGAAGGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240605154632_2hdl CCAACAACTCCCTTCCTGAACCGTCTCCAGGG(+CAGTGT)GATCCCTAAAGCCATTTGAGTCACTGGAATT NGG 10 8 17 8 24 0 100 5 10
    240605154630_5v0x GCAAAACTAACCTAAGGCTAAGTGACCACAGG(+CAGTGT)GATCCTCTGGGTGAACAGTGAGGACTATAAC NGG 10 8 17 8 24 0 100 5 10
    240605154628_ubha CTGTGGTTATAGTCCTCACTGTTCACCCAGAG(+CAGTGT)GATCCCTGTGGTCACTTAGCCTTAGGTTAGT NGG 10 8 17 8 24 0 100 5 10
    240605154627_45yu CTCGGTGCATAACCTTGATCGTGGAGCCTCAG(+CAGTGT)GATCCCAAAACAGGATGTTTTGGGTGTCAAT NGG 10 8 17 8 24 0 100 5 10
    240605154625_x290 ATCAGATTGACACCCAAAACATCCTGTTTTGG(+CAGTGT)GATCCTGAGGCTCCACGATCAAGGTTATGCA NGG 10 8 17 8 24 0 100 5 10
    240605154623_7md9 TGGAAGAGTTGTGTCCTTCCCCTACGAAGGTG(+CAGTGT)GTACCGATGTGCTCTGCCTTTCTGATCCCAT NGG 10 8 17 8 24 0 100 5 10
    240605154622_2p8a ATAAAATGGGATCAGAAAGGCAGAGCACATCG(+CAGTGT)GTACCACCTTCGTAGGGGAAGGACACAACTC NGG 10 8 17 8 24 0 100 5 10