Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154620_b2rm CACACACGTGCATGCTCTGTGCGTGTATGTGG(+CAGTGT)GTACCGTGTATATGTGTGTGAGTGTTGTATA NGG 10 8 17 8 24 0 100 5 10
    240605154619_rm1z ATGCATATACAACACTCACACACATATACACG(+CAGTGT)GTACCCACATACACGCACAGAGCATGCACGT NGG 10 8 17 8 24 0 100 5 10
    240605154617_jgwd CTACTGATACTTTCAGGGGTGGTCCCATGGGG(+CAGTGT)GATCCATGCAGTGGAAGAAACACTGGATTGG NGG 10 8 17 8 24 0 100 5 10
    240605154615_3jp7 CGTACCCAATCCAGTGTTTCTTCCACTGCATG(+CAGTGT)GATCCCCCATGGGACCACCCCTGAAAGTATC NGG 10 8 17 8 24 0 100 5 10
    240605154614_s7i0 ATGTAATTAGGCTTTTTGAGGTAGTTCTCAAG(+CAGTGT)GATCCTCTTTTGACTCTTGGGAAAGAATTGT NGG 10 8 17 8 24 0 100 5 10
    240605154613_4ee5 CACGCACAATTCTTTCCCAAGAGTCAAAAGAG(+CAGTGT)GATCCTTGAGAACTACCTCAAAAAGCCTAAT NGG 10 8 17 8 24 0 100 5 10
    240605154611_y2g2 ATGAGGAGTAATCTCCTGTTATTTTCTATTTG(+CAGTGT)GTACCAAGATAGCTAATATTACGTGAAATTT NGG 10 8 17 8 24 0 100 5 10
    240605154610_9ct1 CCAGAAAATTTCACGTAATATTAGCTATCTTG(+CAGTGT)GTACCAAATAGAAAATAACAGGAGATTACTC NGG 10 8 17 8 24 0 100 5 10
    240605154609_rnuj TTTTCTGGCCCTTTTAAGGGTGCCTACAGTAG(+CAGTGT)GATCCAAAGAGCAGCCCAGCACTCGATTTCT NGG 10 8 17 8 24 0 100 5 10
    240605154608_ctca ATCCTAGAAATCGAGTGCTGGGCTGCTCTTTG(+CAGTGT)GATCCTACTGTAGGCACCCTTAAAAGGGCCA NGG 10 8 17 8 24 0 100 5 10