Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154550_8fha GAACTTGGCCTTTGAAACTCATCATAGACTCG(+CAGTGT)GATCCTTCCTGCATTGTTGCAAGGTACCCAA NGG 10 8 17 8 24 0 100 5 10
    240605154549_cnne GACAATTGGGTACCTTGCAACAATGCAGGAAG(+CAGTGT)GATCCGAGTCTATGATGAGTTTCAAAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605154547_oijj TTAGACCTAATAAAAGCCGAGGGTGAAATCTG(+CAGTGT)GATCCCTGTTTAGCACACCTGAGTTGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240605154546_5f6q TGGGGACAGACAACTCAGGTGTGCTAAACAGG(+CAGTGT)GATCCAGATTTCACCCTCGGCTTTTATTAGG NGG 10 8 17 8 24 0 100 5 10
    240605154544_ew0j TTGAAGCCTGCAGGACCCTTCAACCAACTATG(+CAGTGT)GATCCCTTCGCGCTTCCCTGCTGGCTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240605154543_zla7 ACTGAACAAGAGCCAGCAGGGAAGCGCGAAGG(+CAGTGT)GATCCATAGTTGGTTGAAGGGTCCTGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605154542_p63b ATCCCTTCCCTCACTCACCCTCTGAGATCATG(+CAGTGT)GTACCCACTTGTTAAATGCATACTTCAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605154540_dt6b ATGGCATTTTGAAGTATGCATTTAACAAGTGG(+CAGTGT)GTACCATGATCTCAGAGGGTGAGTGAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605154538_b7m5 ATTTAAGACATTACCTCCTAGCATTCCAAGGG(+CAGTGT)GATCCCAGATTTGAAAGGGCAAGCCTGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605154536_nd00 CATTTATGCCAGGCTTGCCCTTTCAAATCTGG(+CAGTGT)GATCCCCTTGGAATGCTAGGAGGTAATGTCT NGG 10 8 17 8 24 0 100 5 10