Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154445_s2u6 TAGTCCACTGCCAAGTGTGGCCCTAGGCATGG(+CAGTGT)GATCCCGGGGGCTCCAGGAAAATGCTATTGT NGG 10 8 17 8 24 0 100 5 10
    240605154442_y0tb ATGACACAATAGCATTTTCCTGGAGCCCCCGG(+CAGTGT)GATCCCATGCCTAGGGCCACACTTGGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605154441_ttmv AAAAAAACAAACAAAGCAAAAACAAACAAAAG(+CAGTGT)GATCCCCTAGAAACAGTAAGGTAAATCTATT NGG 10 8 17 8 24 0 100 5 10
    240605154439_tnqj AGAAAAATAGATTTACCTTACTGTTTCTAGGG(+CAGTGT)GATCCTTTTGTTTGTTTTTGCTTTGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605154437_hueu TTAGGTAAAATTATAGGAAAAATTATTGAAAG(+CAGTGT)GTACCCTTCAATGGACAGTGTCATCTATTTC NGG 10 8 17 8 24 0 100 5 10
    240605154436_8trz TACTAGAAATAGATGACACTGTCCATTGAAGG(+CAGTGT)GTACCTTTCAATAATTTTTCCTATAATTTTA NGG 10 8 17 8 24 0 100 5 10
    240605154435_co8e TCTAGAAAGCATATTTAAAATCGTTGGTATCG(+CAGTGT)GTACCAGGAGAAGCCAGGCTAAGAAATAAAG NGG 10 8 17 8 24 0 100 5 10
    240605154433_8ep3 CAGGACTTTATTTCTTAGCCTGGCTTCTCCTG(+CAGTGT)GTACCGATACCAACGATTTTAAATATGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605154431_qkss CACAGCCATGCCCAGCTTTTTGTGAGTGCTGG(+CAGTGT)GATCCAAACTCAGGTACTTCTTACCCACTGA NGG 10 8 17 8 24 0 100 5 10
    240605154430_z61v ATGTCTCAGTGGGTAAGAAGTACCTGAGTTTG(+CAGTGT)GATCCCAGCACTCACAAAAAGCTGGGCATGG NGG 10 8 17 8 24 0 100 5 10