Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154342_eak3 GTGCCCTGGTGCTGCAGGATTCCAGACTGTAG(+CAGTGT)GATCCACACAGTGAGCTAACACCCCCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240605154340_p6ju CTTCATCTGAGGGGGTGTTAGCTCACTGTGTG(+CAGTGT)GATCCTACAGTCTGGAATCCTGCAGCACCAG NGG 10 8 17 8 24 0 100 5 10
    240605154338_y1js CAACTTAGAAGAGCAGGCAGTTTAGCCTGGGG(+CAGTGT)GATCCAGACACAGTGGGGCCTGGAAATGGGG NGG 10 8 17 8 24 0 100 5 10
    240605154337_6lpw GCAGACCCCATTTCCAGGCCCCACTGTGTCTG(+CAGTGT)GATCCCCCAGGCTAAACTGCCTGCTCTTCTA NGG 10 8 17 8 24 0 100 5 10
    240605154336_6v7r GGCAGGGCTCATATCTATTGGCAAAATTCAAG(+CAGTGT)GTACCTGTAAGACACTTGCCTGGGCTCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605154334_hb7c ACGGATCCAGAGCCCAGGCAAGTGTCTTACAG(+CAGTGT)GTACCTTGAATTTTGCCAATAGATATGAGCC NGG 10 8 17 8 24 0 100 5 10
    240605154333_fdn0 AAGGTACCTGTAAGACACTTGCCTGGGCTCTG(+CAGTGT)GATCCGTTTCCTGGGTGCCTAGGACCTCACG NGG 10 8 17 8 24 0 100 5 10
    240605154332_ohnv CAGACCGTGAGGTCCTAGGCACCCAGGAAACG(+CAGTGT)GATCCAGAGCCCAGGCAAGTGTCTTACAGGT NGG 10 8 17 8 24 0 100 5 10
    240605154330_o5t4 CCCCAGCAATCCAGTCATCTCCTGTCAGGTTG(+CAGTGT)GATCCAGGAGAAGTGAGGTCAAAACTGAAGA NGG 10 8 17 8 24 0 100 5 10
    240605154329_olon TTGACTCTTCAGTTTTGACCTCACTTCTCCTG(+CAGTGT)GATCCAACCTGACAGGAGATGACTGGATTGC NGG 10 8 17 8 24 0 100 5 10