Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154240_rv3z TAAAGGCAGCTGAGCTGCAGGAGCTCTGACAG(+CAGTGT)GATCCGCACGGCATTCCCAGCTGACCGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605154239_b8u8 TGCAGTCTCCGGTCAGCTGGGAATGCCGTGCG(+CAGTGT)GATCCTGTCAGAGCTCCTGCAGCTCAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240605154238_831f CATCACAGCAATAGTAACCCTAATGAAGACAG(+CAGTGT)GATCCCAGCACCCATGCTGGGTAGCTTACAA NGG 10 8 17 8 24 0 100 5 10
    240605154236_8gvc TGCTGTTGTAAGCTACCCAGCATGGGTGCTGG(+CAGTGT)GATCCTGTCTTCATTAGGGTTACTATTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605154235_k8c3 TAAGAGAGTTTATTCGGGTTCTGCACTCAGAG(+CAGTGT)GTACCTACAACAGACTACAGACAAAATCAAT NGG 10 8 17 8 24 0 100 5 10
    240605154234_opa1 TCCTAATTGATTTTGTCTGTAGTCTGTTGTAG(+CAGTGT)GTACCTCTGAGTGCAGAACCCGAATAAACTC NGG 10 8 17 8 24 0 100 5 10
    240605154232_ljqz GACTTTGTACTCTGGCTGTATCCCATCAGAAG(+CAGTGT)GTACCATTGTAAGACTCTGGTGAGCCATAGC NGG 10 8 17 8 24 0 100 5 10
    240605154231_ir47 GGTAAGCTATGGCTCACCAGAGTCTTACAATG(+CAGTGT)GTACCTTCTGATGGGATACAGCCAGAGTACA NGG 10 8 17 8 24 0 100 5 10
    240605154229_4z6o CTCACCCCTCCCTTGGCATCCCTATAACTAGG(+CAGTGT)GTACCAAGCATGAAGTTCCAGCATGGGGAGT NGG 10 8 17 8 24 0 100 5 10
    240605154228_s8dh GAATAACTCCCCATGCTGGAACTTCATGCTTG(+CAGTGT)GTACCCTAGTTATAGGGATGCCAAGGGAGGG NGG 10 8 17 8 24 0 100 5 10