Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154310_tzlv GGTGTGTTTTTTTTTTTTTCTTTTTCCCCTTG(+CAGTGT)GTACCTATTGGCATTCTGTAAATCACACTAT NGG 10 8 17 8 24 0 100 5 10
    240605154309_3eqf ACCACATAGTGTGATTTACAGAATGCCAATAG(+CAGTGT)GTACCAAGGGGAAAAAGAAAAAAAAAAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605154307_avg0 TTACAGCTGACTTAGTACCCGTGAAATGATTG(+CAGTGT)GTACCACTTGAGCATTGTGAATAATTTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605154306_xhr9 AATAAAGAAAAATTATTCACAATGCTCAAGTG(+CAGTGT)GTACCAATCATTTCACGGGTACTAAGTCAGC NGG 10 8 17 8 24 0 100 5 10
    240605154304_igwy CCTCATTATCTACCAAACACATAAGCCAGTGG(+CAGTGT)GATCCCACAAGCGTCCCTTGCCTTGTCCCCA NGG 10 8 17 8 24 0 100 5 10
    240605154303_1xfz ATATGTGGGGACAAGGCAAGGGACGCTTGTGG(+CAGTGT)GATCCCACTGGCTTATGTGTTTGGTAGATAA NGG 10 8 17 8 24 0 100 5 10
    240605154301_2stx TCCCATTTGTGTTACTGCGACTGAGAAGGCAG(+CAGTGT)GATCCAGGCACCACCTTGAGAGGGAACTGAG NGG 10 8 17 8 24 0 100 5 10
    240605154259_rjp4 TGTGGCTCAGTTCCCTCTCAAGGTGGTGCCTG(+CAGTGT)GATCCTGCCTTCTCAGTCGCAGTAACACAAA NGG 10 8 17 8 24 0 100 5 10
    240605154257_m0vm ACCACCACAGCAGCCTATCAGCCACTTCTGGG(+CAGTGT)GATCCAGTCCAAGAATGAAAGCCCATAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605154256_x2n0 TCTGCAGCCTATGGGCTTTCATTCTTGGACTG(+CAGTGT)GATCCCCAGAAGTGGCTGATAGGCTGCTGTG NGG 10 8 17 8 24 0 100 5 10