Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154227_369a TCAGATTGTCACAGGAATACCCCACTCTGCTG(+CAGTGT)GTACCAATTTGTCTTAGTCAGGGTTTCTATT NGG 10 8 17 8 24 0 100 5 10
    240605154226_qxub GCAGGAATAGAAACCCTGACTAAGACAAATTG(+CAGTGT)GTACCAGCAGAGTGGGGTATTCCTGTGACAA NGG 10 8 17 8 24 0 100 5 10
    240605154224_23xn TTCCTGCCTCTTGCAGAGAGGCTCCAAGTTTG(+CAGTGT)GTACCCATGCCAGCTAGCTGGCAGCTACCTG NGG 10 8 17 8 24 0 100 5 10
    240605154223_qoph GGCTTCAGGTAGCTGCCAGCTAGCTGGCATGG(+CAGTGT)GTACCAAACTTGGAGCCTCTCTGCAAGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605154221_mnj1 ACTGTCTTCACACACACCAGAAGAGGGCATTG(+CAGTGT)GATCCCATTACAGATGTTTGTAAGCTACCAT NGG 10 8 17 8 24 0 100 5 10
    240605154220_8t6e GTTGCATGGTAGCTTACAAACATCTGTAATGG(+CAGTGT)GATCCAATGCCCTCTTCTGGTGTGTGTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605154218_vvv3 GGCCAAGTCATGAGAAGCAAGCCAGTAAGCAG(+CAGTGT)GATCCCTCCATGGCCCCTACATCAGTTCCTA NGG 10 8 17 8 24 0 100 5 10
    240605154216_ipwb GGAGGTAGGAACTGATGTAGGGGCCATGGAGG(+CAGTGT)GATCCTGCTTACTGGCTTGCTTCTCATGACT NGG 10 8 17 8 24 0 100 5 10
    240605154215_59oh CAAAAGTCAATCTCCAACCGTCCAATGTCTGG(+CAGTGT)GATCCAACTTACGATCTTCTGGGCTCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605154214_o084 GCCCTTGGAGGAGCCCAGAAGATCGTAAGTTG(+CAGTGT)GATCCCAGACATTGGACGGTTGGAGATTGAC NGG 10 8 17 8 24 0 100 5 10