Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154157_fah6 CATGAGCCGGGCTGGAAACTTCGTTGATGTGG(+CAGTGT)GATCCAGGCTCCATATGTAGTCCTGGAGGAT NGG 10 8 17 8 24 0 100 5 10
    240605154155_dulw CTGCTATCCTCCAGGACTACATATGGAGCCTG(+CAGTGT)GATCCCACATCAACGAAGTTTCCAGCCCGGC NGG 10 8 17 8 24 0 100 5 10
    240605154154_g3ly CGAGGAGGTCGTGCTAGATGGCCTGTTGGTAG(+CAGTGT)GATCCATGCTGGGGCTGGGCCTCTTGTACTC NGG 10 8 17 8 24 0 100 5 10
    240605154152_iqgf GCTGTGAGTACAAGAGGCCCAGCCCCAGCATG(+CAGTGT)GATCCTACCAACAGGCCATCTAGCACGACCT NGG 10 8 17 8 24 0 100 5 10
    240605154150_03je AGGTGATGTAAGGCCAGCAAGTTGGTTCCTGG(+CAGTGT)GATCCCCAGGTCTGGCATAGTTGGATAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605154147_xjyd AATGCCAACTATCCAACTATGCCAGACCTGGG(+CAGTGT)GATCCCAGGAACCAACTTGCTGGCCTTACAT NGG 10 8 17 8 24 0 100 5 10
    240605154146_13yv GATGAAGGGGATCCTTCCACGCACGCACGAAG(+CAGTGT)GTACCCTCTGTCTATCCTGGGACCTGACCCC NGG 10 8 17 8 24 0 100 5 10
    240605154144_j13g ACTGTGGGGTCAGGTCCCAGGATAGACAGAGG(+CAGTGT)GTACCTTCGTGCGTGCGTGGAAGGATCCCCT NGG 10 8 17 8 24 0 100 5 10
    240605154142_hb03 GTTGAGACTAGTGTTACTTTGTAGATGAAGGG(+CAGTGT)GATCCTTCCACGCACGCACGAAGGTACCCTC NGG 10 8 17 8 24 0 100 5 10
    240605154141_37yj AGACAGAGGGTACCTTCGTGCGTGCGTGGAAG(+CAGTGT)GATCCCCTTCATCTACAAAGTAACACTAGTC NGG 10 8 17 8 24 0 100 5 10