Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154122_wwaw TCCTGGCACACAGTAGGTGGCTGTCCCCCGTG(+CAGTGT)GTACCACAGTACAGACGGAAGGCGTTCTTGA NGG 10 8 17 8 24 0 100 5 10
    240605154121_3r9p GAGTGTCAAGAACGCCTTCCGTCTGTACTGTG(+CAGTGT)GTACCACGGGGGACAGCCACCTACTGTGTGC NGG 10 8 17 8 24 0 100 5 10
    240605154119_9s0q ACTGGCATGAGTTTCGGCATCTTTGGCAGCAG(+CAGTGT)GTACCTTTACCTGCCAAGCCATCTCTACCTT NGG 10 8 17 8 24 0 100 5 10
    240605154118_yo4d CGAATAAGGTAGAGATGGCTTGGCAGGTAAAG(+CAGTGT)GTACCTGCTGCCAAAGATGCCGAAACTCATG NGG 10 8 17 8 24 0 100 5 10
    240605154117_p6kv CCCAGCTTCCTGCTTTGTATGAATTGAGTGAG(+CAGTGT)GTACCTCTGTCCCTGGTTTCCCTCGCACAAG NGG 10 8 17 8 24 0 100 5 10
    240605154116_g4yr CAGTGCTTGTGCGAGGGAAACCAGGGACAGAG(+CAGTGT)GTACCTCACTCAATTCATACAAAGCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605154114_05yt CAAGTGTGTGGAGCGCAGAGAACCCCTGGCAG(+CAGTGT)GATCCTCACCACCAAAGGGTCCTGGAGACTG NGG 10 8 17 8 24 0 100 5 10
    240605154113_axkd CATGCCAGTCTCCAGGACCCTTTGGTGGTGAG(+CAGTGT)GATCCTGCCAGGGGTTCTCTGCGCTCCACAC NGG 10 8 17 8 24 0 100 5 10
    240605154111_cygy TACTCTACTCCATACCCAGCAGCCCTAGAAGG(+CAGTGT)GATCCTGGCCTGCTTTACAGAGAAATGAATG NGG 10 8 17 8 24 0 100 5 10
    240605154109_7ph4 AACTTCATTCATTTCTCTGTAAAGCAGGCCAG(+CAGTGT)GATCCCTTCTAGGGCTGCTGGGTATGGAGTA NGG 10 8 17 8 24 0 100 5 10