Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605154020_m78q AGCCTCCACACGAGTGTTCTAGTACATGTTAG(+CAGTGT)GATCCTCTCCTGTACCAGTGTCCAGGTGTAA NGG 10 8 17 8 24 0 100 5 10
    240605154019_zpdl GTCTGTTACACCTGGACACTGGTACAGGAGAG(+CAGTGT)GATCCTAACATGTACTAGAACACTCGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605154018_bhhn TTCCACTCAAATCAGTTGAGAGCCCAAATCAG(+CAGTGT)GATCCACATATCATTCATTGTCATTAGATCT NGG 10 8 17 8 24 0 100 5 10
    240605154016_km50 TATTGAGATCTAATGACAATGAATGATATGTG(+CAGTGT)GATCCTGATTTGGGCTCTCAACTGATTTGAG NGG 10 8 17 8 24 0 100 5 10
    240605154015_vfst GCTGTTAACAGGAGGTCCAAACCTCTTGGAAG(+CAGTGT)GTACCAAGCAGCTAATAAAGGCTGGTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605154013_x9jr GATGAAGAAGACCAGCCTTTATTAGCTGCTTG(+CAGTGT)GTACCTTCCAAGAGGTTTGGACCTCCTGTTA NGG 10 8 17 8 24 0 100 5 10
    240605154012_rgvb AGTCTCCCTAGCGCCACTGTGCTGAGAACAGG(+CAGTGT)GATCCTCTGGATGAGGAAGCCCTGGCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240605154010_yxcg CTGAGCTGGGGCCAGGGCTTCCTCATCCAGAG(+CAGTGT)GATCCCTGTTCTCAGCACAGTGGCGCTAGGG NGG 10 8 17 8 24 0 100 5 10
    240605154008_cnc7 CATCTAACAGCAGCTTGAACCTTGGATCTTAG(+CAGTGT)GATCCGCTTGACATAGTCTGCTTTCTCGGAG NGG 10 8 17 8 24 0 100 5 10
    240605154007_g8d2 AAGTTCTCCGAGAAAGCAGACTATGTCAAGCG(+CAGTGT)GATCCTAAGATCCAAGGTTCAAGCTGCTGTT NGG 10 8 17 8 24 0 100 5 10