Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605153919_gipi TGAAATTTTCATTTTGTTTAAAAAACAATACG(+CAGTGT)GTACCTTGTTCCCTAAAACAGTCTGCACTTA NGG 10 8 17 8 24 0 100 5 10
    240605153918_4zq6 ACTTCTAAGTGCAGACTGTTTTAGGGAACAAG(+CAGTGT)GTACCGTATTGTTTTTTAAACAAAATGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605153916_5xsn ATAAAGGTTCAGGTGACAGGAACAGAAATCCG(+CAGTGT)GTACCCCAGGGGCTCTAAATAACACCGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605153913_ulx6 ACTGCTCCACGGTGTTATTTAGAGCCCCTGGG(+CAGTGT)GTACCGGATTTCTGTTCCTGTCACCTGAACC NGG 10 8 17 8 24 0 100 5 10
    240605153911_lb74 TGTGGCTGGCATTTACATGGATGTGAGGGGAG(+CAGTGT)GATCCGAACTCAAATCTTCACATATGGATGG NGG 10 8 17 8 24 0 100 5 10
    240605153910_ces0 GCTTGCCATCCATATGTGAAGATTTGAGTTCG(+CAGTGT)GATCCTCCCCTCACATCCATGTAAATGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605153908_yoai CTACCGACAGTAAAAGTTAAAAGCAACACCAG(+CAGTGT)GTACCTGCATCCATTTACAATACTTGCAAAT NGG 10 8 17 8 24 0 100 5 10
    240605153907_jwjl CTGTGATTTGCAAGTATTGTAAATGGATGCAG(+CAGTGT)GTACCTGGTGTTGCTTTTAACTTTTACTGTC NGG 10 8 17 8 24 0 100 5 10
    240605153904_q7rq CAATTTTACAAATAAATACTTTCTTTCCTTGG(+CAGTGT)GTACCTAGAATAATTAATCCAGCATACAACT NGG 10 8 17 8 24 0 100 5 10
    240605153903_sv7k TTCAAAGTTGTATGCTGGATTAATTATTCTAG(+CAGTGT)GTACCCAAGGAAAGAAAGTATTTATTTGTAA NGG 10 8 17 8 24 0 100 5 10