Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605153821_6ku8 TTACTCTTAATTACTGTGCTCACAAAGCCTCG(+CAGTGT)GATCCTTGGATCTCTTGCATCTGTGACTAAC NGG 10 8 17 8 24 0 100 5 10
    240605153819_52mw CTTAGAATTCTCAATGGAAGAGAATGGTACAG(+CAGTGT)GTACCAACAACATGCCATATGCATAGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605153818_5qig CTGTGCTGCTCTATGCATATGGCATGTTGTTG(+CAGTGT)GTACCTGTACCATTCTCTTCCATTGAGAATT NGG 10 8 17 8 24 0 100 5 10
    240605153817_pa7g ATGTAATAGTGTCCAGCCAAACAATCCAACAG(+CAGTGT)GATCCTCCACTCTTCTTTCATGTAAGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240605153815_teaf TAACTTACAACTTACATGAAAGAAGAGTGGAG(+CAGTGT)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    240605134958_dkoy TAGAAACCCACACTTGGAAATACTTCCCGTGG(+CAGTGT)GATCCTGGCAGCTGCCTCTCCGAGCTTTACT NGG 10 8 17 8 24 0 100 5 10
    240605134956_qlfu TGCCACCACCAGAGTCCATGCACCTCAACGTG(+CAGTGT)GATCCACTTGGGTCCTGGCATCTTGAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240605134955_wy41 ATAAATCACCTCAAGATGCCAGGACCCAAGTG(+CAGTGT)GATCCACGTTGAGGTGCATGGACTCTGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605134952_jp5x TTCTGCTTCTTACCCTCCCTTCCAAGCCCTGG(+CAGTGT)GATCCTAAAAGTCCCGCCTTTGTCTGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605134950_ukp8 CTAGGCAGGACAGACAAAGGCGGGACTTTTAG(+CAGTGT)GATCCCAGGGCTTGGAAGGGAGGGTAAGAAG NGG 10 8 17 8 24 0 100 5 10