Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134906_fwml GGAGGTGACCTCAAGGACTCTGTGGGGATTTG(+CAGTGT)GATCCGGCTGGGCTCCGGCTTGAGAGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605134904_kl91 ATTTTCCAGCCTCTCAAGCCGGAGCCCAGCCG(+CAGTGT)GATCCAAATCCCCACAGAGTCCTTGAGGTCA NGG 10 8 17 8 24 0 100 5 10
    240605134903_k3dc CGACTGAGACAGGTCAGAATCGAGCCAGCTAG(+CAGTGT)GATCCGTCTGTGCCTAGGGAGGAAATGGGGT NGG 10 8 17 8 24 0 100 5 10
    240605134901_kqld GCATTACCCCATTTCCTCCCTAGGCACAGACG(+CAGTGT)GATCCTAGCTGGCTCGATTCTGACCTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240605134900_3rmv GGCCACAGAGAGGTGGGAAGGATTTGTTGAGG(+CAGTGT)GATCCCAGGATCAAAGGAGACTGCCATGACC NGG 10 8 17 8 24 0 100 5 10
    240605134857_twu8 CCTTTGGTCATGGCAGTCTCCTTTGATCCTGG(+CAGTGT)GATCCCTCAACAAATCCTTCCCACCTCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605134855_nhyh ACGAAGGGCCAGCTTGAGGAAAGCCTCAGGTG(+CAGTGT)GTACCAAAATTAGCTGCCGTCTGCCCCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605134854_iq3e CACCAGCAGGGGGCAGACGGCAGCTAATTTTG(+CAGTGT)GTACCACCTGAGGCTTTCCTCAAGCTGGCCC NGG 10 8 17 8 24 0 100 5 10
    240605134852_44b2 TAGGGAACAGGGAAATCTTTGGTCTGGGGCTG(+CAGTGT)GATCCTGGAGCTCAAGTCCAGGGAAGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605134850_1zwf CCTTCCCCTGCTTCCCTGGACTTGAGCTCCAG(+CAGTGT)GATCCAGCCCCAGACCAAAGATTTCCCTGTT NGG 10 8 17 8 24 0 100 5 10