Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134835_hm2f GGAAGGGCTGGGGGAGGGGGAGTCAGTGCGAG(+CAGTGT)GTACCTCGAAGGCTATTGCTTTCTAGGGATT NGG 10 8 17 8 24 0 100 5 10
    240605134834_vydn GTGAAAATCCCTAGAAAGCAATAGCCTTCGAG(+CAGTGT)GTACCTCGCACTGACTCCCCCTCCCCCAGCC NGG 10 8 17 8 24 0 100 5 10
    240605134832_jrf2 TCTAGAAGATCCCTGTGCCCCCAGGGGAGGCG(+CAGTGT)GATCCTAAGGGGTGAGGGATAAGGATTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605134831_7tk6 CCATGCCCCAATCCTTATCCCTCACCCCTTAG(+CAGTGT)GATCCGCCTCCCCTGGGGGCACAGGGATCTT NGG 10 8 17 8 24 0 100 5 10
    240605134829_8sci AAAAAACTCAGGGTGAGAGGAACGAGGAAGGG(+CAGTGT)GATCCAATTTAGCTCAGCATCCTAAAGTGTT NGG 10 8 17 8 24 0 100 5 10
    240605134827_jimz AAGAAAACACTTTAGGATGCTGAGCTAAATTG(+CAGTGT)GATCCCCTTCCTCGTTCCTCTCACCCTGAGT NGG 10 8 17 8 24 0 100 5 10
    240605134826_0r3r CTTTTCAGATAGCCCTGGCTGGTGGGGGAATG(+CAGTGT)GATCCCTGAGAACACGGGCATGTCACACAGG NGG 10 8 17 8 24 0 100 5 10
    240605134824_t1c2 CATTCCCTGTGTGACATGCCCGTGTTCTCAGG(+CAGTGT)GATCCATTCCCCCACCAGCCAGGGCTATCTG NGG 10 8 17 8 24 0 100 5 10
    240605134822_kwba GCAGGGGGAGAGAGGAGCTGAGGAAATGTGGG(+CAGTGT)GATCCTGGGGGCTCGGACCGGGGTGAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240605134819_vfti ACATAGACACTCACCCCGGTCCGAGCCCCCAG(+CAGTGT)GATCCCCACATTTCCTCAGCTCCTCTCTCCC NGG 10 8 17 8 24 0 100 5 10