Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134849_neni TCTCAGGAGGTCCCTTCTCAAGGACACGCGGG(+CAGTGT)GTACCTCTCACCCAACTACCAACAGCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605134847_2yky TGGCTTTCCTGCTGTTGGTAGTTGGGTGAGAG(+CAGTGT)GTACCCCGCGTGTCCTTGAGAAGGGACCTCC NGG 10 8 17 8 24 0 100 5 10
    240605134846_jb95 AGAAGCAGGAGGGTGTGGGTTGTGGGTGACAG(+CAGTGT)GTACCTCACAGGGTCTCAATTATCGCTTGAC NGG 10 8 17 8 24 0 100 5 10
    240605134845_lpcn GCAGAGTCAAGCGATAATTGAGACCCTGTGAG(+CAGTGT)GTACCTGTCACCCACAACCCACACCCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605134843_hylh GAGAGAATGTCTCTCACCCACCGGGAGTCAGG(+CAGTGT)GATCCATCAACGAGTGGGGCTGGGGGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605134841_tvqf TCTATTCCTGCCCCCAGCCCCACTCGTTGATG(+CAGTGT)GATCCCTGACTCCCGGTGGGTGAGAGACATT NGG 10 8 17 8 24 0 100 5 10
    240605134840_p69g GTCTGGGCTGCCAGCATTTCCCAGTGTTTCAG(+CAGTGT)GATCCTGAAGGCACAGAAAACGTTGAGATCG NGG 10 8 17 8 24 0 100 5 10
    240605134839_9rgb TCCCCCGATCTCAACGTTTTCTGTGCCTTCAG(+CAGTGT)GATCCTGAAACACTGGGAAATGCTGGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240605134838_6vql AAAAAAAAAAAAATACCGTGGGAAAACAAGTG(+CAGTGT)GTACCGTAATATTCCGAGTATAGGTCCCCAA NGG 10 8 17 8 24 0 100 5 10
    240605134836_gvte AATGGTTGGGGACCTATACTCGGAATATTACG(+CAGTGT)GTACCACTTGTTTTCCCACGGTATTTTTTTT NGG 10 8 17 8 24 0 100 5 10