Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134818_b31z CTATAGATGGAGATCTGAAGATTAGACTCTAG(+CAGTGT)GATCCACAGATGAGAGAGTATACGTGACATT NGG 10 8 17 8 24 0 100 5 10
    240605134816_7mu2 AGGCAAATGTCACGTATACTCTCTCATCTGTG(+CAGTGT)GATCCTAGAGTCTAATCTTCAGATCTCCATC NGG 10 8 17 8 24 0 100 5 10
    240605134815_1guy CCTCACCAGGTAGCTCTCTAGCCCCAAGAAAG(+CAGTGT)GATCCCACCAGAAACAGTATCAGATGTCATA NGG 10 8 17 8 24 0 100 5 10
    240605134813_32fn TAATTTATGACATCTGATACTGTTTCTGGTGG(+CAGTGT)GATCCTTTCTTGGGGCTAGAGAGCTACCTGG NGG 10 8 17 8 24 0 100 5 10
    240605134811_l5el CAAGCTGCTGAAACCTTTAAAATGTATCAAGG(+CAGTGT)GATCCATAGCCTCTACCATCCGGAATGCTAA NGG 10 8 17 8 24 0 100 5 10
    240605134810_27hx ATCCTTTAGCATTCCGGATGGTAGAGGCTATG(+CAGTGT)GATCCCTTGATACATTTTAAAGGTTTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605134809_qgu5 TAATAGTTGCAATGTAATTTATGGAGCACTTG(+CAGTGT)GTACCACATTGTGTCTATTCTTTCAAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240605134807_3orj CACAAGCAGCTTGAAAGAATAGACACAATGTG(+CAGTGT)GTACCAAGTGCTCCATAAATTACATTGCAAC NGG 10 8 17 8 24 0 100 5 10
    240605134805_b830 ACATTGTCTGTGTACATTCTTTCAAACTCCTG(+CAGTGT)GTACCCTCCACACACTGCTCTGCTTACTATT NGG 10 8 17 8 24 0 100 5 10
    240605134804_mmam GCAACAATAGTAAGCAGAGCAGTGTGTGGAGG(+CAGTGT)GTACCAGGAGTTTGAAAGAATGTACACAGAC NGG 10 8 17 8 24 0 100 5 10