Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134730_mvv5 GACCTGCATCCCCTAAGTGAGGACCCTACAGG(+CAGTGT)GTACCTTGTGCTCTGCCCCCAGACCCATAGC NGG 10 8 17 8 24 0 100 5 10
    240605134729_zhgu CTAAGGCTATGGGTCTGGGGGCAGAGCACAAG(+CAGTGT)GTACCCTGTAGGGTCCTCACTTAGGGGATGC NGG 10 8 17 8 24 0 100 5 10
    240605134727_nwio TCCTTGGGGGTCTGGCAGGTGGCCACACCACG(+CAGTGT)GATCCTTGAGCTGTCTCGAGGGTCAGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605134726_bf2g TGCCACTGGCCTGACCCTCGAGACAGCTCAAG(+CAGTGT)GATCCGTGGTGTGGCCACCTGCCAGACCCCC NGG 10 8 17 8 24 0 100 5 10
    240605134723_yr0h GCTGGCTGCTCTCCTCCCTGCAGTCCACCCTG(+CAGTGT)GATCCCTCGCCTCTAGGGAACAGAAGTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605134722_dv7q CCAGCGTAAACTTCTGTTCCCTAGAGGCGAGG(+CAGTGT)GATCCAGGGTGGACTGCAGGGAGGAGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605134719_8qda CTCTAAGTTGCTCTGACTAGCCTTGAGCCCAG(+CAGTGT)GATCCTCATGAAGGCTGAGATTATAAATATA NGG 10 8 17 8 24 0 100 5 10
    240605134718_yr9v GAATTTATATTTATAATCTCAGCCTTCATGAG(+CAGTGT)GATCCTGGGCTCAAGGCTAGTCAGAGCAACT NGG 10 8 17 8 24 0 100 5 10
    240605134716_q5cf CACCTCTAACCCCAGCACTGAGGGAATAGGTG(+CAGTGT)GATCCAAGGGGTTCTCTGGACAAGTGGTCTA NGG 10 8 17 8 24 0 100 5 10
    240605134714_9z2a TGGGCTAGACCACTTGTCCAGAGAACCCCTTG(+CAGTGT)GATCCACCTATTCCCTCAGTGCTGGGGTTAG NGG 10 8 17 8 24 0 100 5 10