Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134624_k154 GTGACGCTCAGATCTGAGAAGAAACATGATGG(+CAGTGT)GATCCATATCAGGTTGTGAATATTTTTTTGA NGG 10 8 17 8 24 0 100 5 10
    240605134623_z6gg TGTTCTCAAAAAAATATTCACAACCTGATATG(+CAGTGT)GATCCCATCATGTTTCTTCTCAGATCTGAGC NGG 10 8 17 8 24 0 100 5 10
    240605134622_5fd2 TAAAGTACTATGACACTAGAACCTTTGCAGAG(+CAGTGT)GATCCAACTACAATGTAAGGTTGATCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240605134621_jy2p GATACTGGAAGATCAACCTTACATTGTAGTTG(+CAGTGT)GATCCTCTGCAAAGGTTCTAGTGTCATAGTA NGG 10 8 17 8 24 0 100 5 10
    240605134618_t0w8 AGAGCGGTCACTGGACTAGGATGCATCCCTTG(+CAGTGT)GATCCTGGGATAGGCTCTGATCGGACCATAA NGG 10 8 17 8 24 0 100 5 10
    240605134616_fz47 AACACTTATGGTCCGATCAGAGCCTATCCCAG(+CAGTGT)GATCCAAGGGATGCATCCTAGTCCAGTGACC NGG 10 8 17 8 24 0 100 5 10
    240605134614_k2n2 TTTTAAGACTAGCCACAGGCTATGAATATATG(+CAGTGT)GTACCCCTTTGAAGGTAAAAATTGAAGCAAT NGG 10 8 17 8 24 0 100 5 10
    240605134612_bpwx AAAACATTGCTTCAATTTTTACCTTCAAAGGG(+CAGTGT)GTACCATATATTCATAGCCTGTGGCTAGTCT NGG 10 8 17 8 24 0 100 5 10
    240605134611_tgdi GATTAAATTCGGGTCATCAGGATTGGCAAGAG(+CAGTGT)GTACCTTTACCTGCTGAGTCATTGCATTGGC NGG 10 8 17 8 24 0 100 5 10
    240605134610_2d3v ATTAGGCCAATGCAATGACTCAGCAGGTAAAG(+CAGTGT)GTACCTCTTGCCAATCCTGATGACCCGAATT NGG 10 8 17 8 24 0 100 5 10