Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134518_y6kx GGTCCAAACAACACATCATCATGATGTCCCAG(+CAGTGT)GATCCACTATGTCCTCATTGCTTCCCAGAAT NGG 10 8 17 8 24 0 100 5 10
    240605134517_6i2p GAAGGATTCTGGGAAGCAATGAGGACATAGTG(+CAGTGT)GATCCTGGGACATCATGATGATGTGTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240605134516_ecv1 GCTCAGGAAAGGGTGATAATGGATAGGATGCG(+CAGTGT)GATCCCTAAGTAGATGGGATAAATGTATTAC NGG 10 8 17 8 24 0 100 5 10
    240605134514_cb74 CGTGTGTAATACATTTATCCCATCTACTTAGG(+CAGTGT)GATCCGCATCCTATCCATTATCACCCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605134512_8c4f ACCAGCCCAAGACATTTTTACTTTTCTCCTGG(+CAGTGT)GATCCAAAGAAATTCTGTGCTTCATGAGAAC NGG 10 8 17 8 24 0 100 5 10
    240605134511_rsbg TCTTAGTTCTCATGAAGCACAGAATTTCTTTG(+CAGTGT)GATCCCAGGAGAAAAGTAAAAATGTCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240605134509_0j0q ACACACATTTATATGCTATATCTGTATTAAGG(+CAGTGT)GATCCTCCAGGGTTGCCCTATTCTTCACCTC NGG 10 8 17 8 24 0 100 5 10
    240605134507_p68q CACAGGAGGTGAAGAATAGGGCAACCCTGGAG(+CAGTGT)GATCCCTTAATACAGATATAGCATATAAATG NGG 10 8 17 8 24 0 100 5 10
    240605134506_9kzf GGAGTATGCCGAAAGGATCGGAGTCTAAACTG(+CAGTGT)GTACCCTAGCATTTCAAAGGTCCTCAACAGA NGG 10 8 17 8 24 0 100 5 10
    240605134504_2sds AAGTATCTGTTGAGGACCTTTGAAATGCTAGG(+CAGTGT)GTACCAGTTTAGACTCCGATCCTTTCGGCAT NGG 10 8 17 8 24 0 100 5 10