Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134409_69de AACACTCCCTCAGTCCCAGAAGTTTCCCCACG(+CAGTGT)GATCCCCATTTGCATATAACCTCTTCCCATC NGG 10 8 17 8 24 0 100 5 10
    240605134407_2lwj GGTAGGATGGGAAGAGGTTATATGCAAATGGG(+CAGTGT)GATCCGTGGGGAAACTTCTGGGACTGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605134406_hxjb AGGAAATGGGAGGGAGATGAGAAAACAGGATG(+CAGTGT)GTACCAATCACCTGGGTAATCAACTGGTTGA NGG 10 8 17 8 24 0 100 5 10
    240605134405_ebfi CCTCTTCAACCAGTTGATTACCCAGGTGATTG(+CAGTGT)GTACCATCCTGTTTTCTCATCTCCCTCCCAT NGG 10 8 17 8 24 0 100 5 10
    240605134403_8efp ACTACATGGTGGCTTGCAATCATCTGTAATGG(+CAGTGT)GATCCAATGCCTTCTTTGGGTGAATCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605134402_yzzm GCTATCTTCAGATTCACCCAAAGAAGGCATTG(+CAGTGT)GATCCCATTACAGATGATTGCAAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605134400_hyc1 TTTTTTTTTTTTTGGTGTGTGTATTGAAATTG(+CAGTGT)GATCCATGATTTTGCATATGCTAGGCAAGTA NGG 10 8 17 8 24 0 100 5 10
    240605134359_kn6j AGTACTACTTGCCTAGCATATGCAAAATCATG(+CAGTGT)GATCCAATTTCAATACACACACCAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605134356_r4ot ACTAGGCCTGATAATCTTAGTTCAATCCCTGG(+CAGTGT)GATCCATACAGTTGTGGGAGAGAAGCAACTT NGG 10 8 17 8 24 0 100 5 10
    240605134355_aory TACAGAAGTTGCTTCTCTCCCACAACTGTATG(+CAGTGT)GATCCCAGGGATTGAACTAAGATTATCAGGC NGG 10 8 17 8 24 0 100 5 10