Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134443_mjji ATCAGTATCCAGAAGCAGAGTGGTTGCCCCAG(+CAGTGT)GATCCCACCTCACGTGTGATGCTACCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605134441_dnky AGGAGTGCTGGGTAGCATCACACGTGAGGTGG(+CAGTGT)GATCCTGGGGCAACCACTCTGCTTCTGGATA NGG 10 8 17 8 24 0 100 5 10
    240605134440_a600 ATGCCCAGGAAAAATCCAGATCTCCATAGCTG(+CAGTGT)GATCCTGGGTGGCTGGCTGGCGCAGGCATCT NGG 10 8 17 8 24 0 100 5 10
    240605134437_ayqh GCCATAGATGCCTGCGCCAGCCAGCCACCCAG(+CAGTGT)GATCCAGCTATGGAGATCTGGATTTTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605134435_lmga GGGAATCTGGTCCCTCCTTGTGAGTTCCTGAG(+CAGTGT)GATCCAACTCAGTTAGGCTTGGTAGCGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605134434_e049 GAAGGCCCTCGCTACCAAGCCTAACTGAGTTG(+CAGTGT)GATCCTCAGGAACTCACAAGGAGGGACCAGA NGG 10 8 17 8 24 0 100 5 10
    240605134433_ebso GCTGTTTTCAGACACACCAGAAGAGGGCATCG(+CAGTGT)GATCCCATCACACATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605134431_1ugm ACCACATGGTGGCTCACAACCATGTGTGATGG(+CAGTGT)GATCCGATGCCCTCTTCTGGTGTGTCTGAAA NGG 10 8 17 8 24 0 100 5 10
    240605134429_m1s8 GGCTCACAATGCCTGTACCTCCAGCTTTGAGG(+CAGTGT)GATCCTTTATTCAGGTGTACTTGGACACATG NGG 10 8 17 8 24 0 100 5 10
    240605134428_cloc GTCTACATGTGTCCAAGTACACCTGAATAAAG(+CAGTGT)GATCCCTCAAAGCTGGAGGTACAGGCATTGT NGG 10 8 17 8 24 0 100 5 10