Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134536_6nh4 AGACTCAGGAAAGAGGTACTTGGGGTCTGTGG(+CAGTGT)GTACCCAGATATATCTAAAAATACATAGACA NGG 10 8 17 8 24 0 100 5 10
    240605134534_0h3g CCAGCTGTCTATGTATTTTTAGATATATCTGG(+CAGTGT)GTACCCACAGACCCCAAGTACCTCTTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605134533_gtar TCAACTGACTACGTAGCTGACCTAGAGCTTTG(+CAGTGT)GATCCTCCACCTCCACTTCCCCAGTGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240605134532_ijz9 TACAGGCCAGCACTGGGGAAGTGGAGGTGGAG(+CAGTGT)GATCCAAAGCTCTAGGTCAGCTACGTAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605134530_nxoe CACCATTTGAGGCACAAAATCCAAACTTACAG(+CAGTGT)GATCCGATATTATGTTGATCTGACAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605134529_t9ds AGCCCAGAAACTGTCAGATCAACATAATATCG(+CAGTGT)GATCCTGTAAGTTTGGATTTTGTGCCTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605134528_h7wa TGCTAAGCAGAATCAGGCTGTAGGAAGGCATG(+CAGTGT)GATCCTGGGGCAGGAACTGCACAGCCAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605134524_5mef TCAGGGCCTTGGCTGTGCAGTTCCTGCCCCAG(+CAGTGT)GATCCATGCCTTCCTACAGCCTGATTCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605134523_lnad GACGTTGATGGATGACTTTTCCTTCATCCATG(+CAGTGT)GATCCCAAGAGTGGTGCAGTCCGCCAGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605134521_23wy GCGGTTCTCCTGGCGGACTGCACCACTCTTGG(+CAGTGT)GATCCATGGATGAAGGAAAAGTCATCCATCA NGG 10 8 17 8 24 0 100 5 10