Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134353_5ca5 GAAGACCCAGGTTCATTGCCAGCACCCACATG(+CAGTGT)GTACCTCACACCCACCTATTACTACAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240605134352_65zs CCTCTGGAACTGTAGTAATAGGTGGGTGTGAG(+CAGTGT)GTACCATGTGGGTGCTGGCAATGAACCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605134351_nxeq TGTAGCTGAAAACCCAAGCCATGGCCTCTCAG(+CAGTGT)GATCCGTGTGCAATCTCAGGGCCCTGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605134350_q1ps GGCAAGAGCCCAGGGCCCTGAGATTGCACACG(+CAGTGT)GATCCTGAGAGGCCATGGCTTGGGTTTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605134348_cmm1 CATGCTTGCTTGGTCCAATTACTTGTGTGTTG(+CAGTGT)GATCCAAGATGTATTTTAAACAAATATTGGC NGG 10 8 17 8 24 0 100 5 10
    240605134347_3xrh TCTATGCCAATATTTGTTTAAAATACATCTTG(+CAGTGT)GATCCAACACACAAGTAATTGGACCAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240605134346_2ssy AATTGTAACATAGGTAGTATGCCTTCACACTG(+CAGTGT)GATCCCGGGGGCATGGGTTTTGGAGATGTTA NGG 10 8 17 8 24 0 100 5 10
    240605134343_ib86 CAAAATAACATCTCCAAAACCCATGCCCCCGG(+CAGTGT)GATCCAGTGTGAAGGCATACTACCTATGTTA NGG 10 8 17 8 24 0 100 5 10
    240605134341_fhrf CACCACCTTGCTACGTGCATGTACCAGGCAAG(+CAGTGT)GTACCAAAGATAACAAATGAAAAACAAACCC NGG 10 8 17 8 24 0 100 5 10
    240605134340_jomz ATCATGGGTTTGTTTTTCATTTGTTATCTTTG(+CAGTGT)GTACCTTGCCTGGTACATGCACGTAGCAAGG NGG 10 8 17 8 24 0 100 5 10