Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134257_p37a CATTTGCTTTCCTTTGCCCTTAAATAAATATG(+CAGTGT)GTACCAGAAAGCCCACATTGTGAGTTGTCTT NGG 10 8 17 8 24 0 100 5 10
    240605134255_dddq TGCATAAGACAACTCACAATGTGGGCTTTCTG(+CAGTGT)GTACCATATTTATTTAAGGGCAAAGGAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605134254_6i8s ACCAAATATACAGCATCAATAAAATGTACACG(+CAGTGT)GTACCATGAAAAATTCGAGAACTCAAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240605134253_5d4f GAAAAGCAGCTTGAGTTCTCGAATTTTTCATG(+CAGTGT)GTACCGTGTACATTTTATTGATGCTGTATAT NGG 10 8 17 8 24 0 100 5 10
    240605134251_thii ACTGCTGGTGTATCTGTGAAGTGTTTGCAGTG(+CAGTGT)GATCCATCTGCCGCTGCCTGCTAACATATGA NGG 10 8 17 8 24 0 100 5 10
    240605134250_9y1b TTAGTTCATATGTTAGCAGGCAGCGGCAGATG(+CAGTGT)GATCCACTGCAAACACTTCACAGATACACCA NGG 10 8 17 8 24 0 100 5 10
    240605134248_afjc GTTCCAGGAGCCTCCTCATGTTTGCCTGCTGG(+CAGTGT)GATCCCTGCACAAGGTGTTTCTTTACAATAG NGG 10 8 17 8 24 0 100 5 10
    240605134247_vhkn CTGGTCTATTGTAAAGAAACACCTTGTGCAGG(+CAGTGT)GATCCCAGCAGGCAAACATGAGGAGGCTCCT NGG 10 8 17 8 24 0 100 5 10
    240605134245_s5jy AATACTACCATAAATAACTAGCACCCACCAAG(+CAGTGT)GATCCCAAGTTCCTGTCTTTTCCAAATGGCT NGG 10 8 17 8 24 0 100 5 10
    240605134243_smxb ACCCTAGCCATTTGGAAAAGACAGGAACTTGG(+CAGTGT)GATCCTTGGTGGGTGCTAGTTATTTATGGTA NGG 10 8 17 8 24 0 100 5 10