Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134325_1y98 TTGGCACAAACTGGAGGATTTTACTGTCCATG(+CAGTGT)GTACCTTTGCTGGATCCTTTCCCAAAGACAG NGG 10 8 17 8 24 0 100 5 10
    240605134323_fsmc TAACACTGTCTTTGGGAAAGGATCCAGCAAAG(+CAGTGT)GTACCATGGACAGTAAAATCCTCCAGTTTGT NGG 10 8 17 8 24 0 100 5 10
    240605134322_y5gg TATGTAGAATATACTGGTCTCCAACTCACAAG(+CAGTGT)GATCCACCTGCCTCTGCCTCCTGAGGGGTGA NGG 10 8 17 8 24 0 100 5 10
    240605134320_x1xl CAGTCTCACCCCTCAGGAGGCAGAGGCAGGTG(+CAGTGT)GATCCTTGTGAGTTGGAGACCAGTATATTCT NGG 10 8 17 8 24 0 100 5 10
    240605134319_oz68 TGAGCTGAGCGTCTCTATCCTTCCCTGTTCAG(+CAGTGT)GATCCTTCCTGCAGGTGCTCATGAGATAGTA NGG 10 8 17 8 24 0 100 5 10
    240605134317_oflr GATGCTACTATCTCATGAGCACCTGCAGGAAG(+CAGTGT)GATCCTGAACAGGGAAGGATAGAGACGCTCA NGG 10 8 17 8 24 0 100 5 10
    240605134316_6vlv CCAGTCTACACAGACAGGCCCCATGGATTCTG(+CAGTGT)GTACCTTCAGTTATGGGAGTTATTTGTAATT NGG 10 8 17 8 24 0 100 5 10
    240605134315_h301 ACACAAATTACAAATAACTCCCATAACTGAAG(+CAGTGT)GTACCAGAATCCATGGGGCCTGTCTGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240605134313_3zqm CTAGGCAGCTGTATAGAGAAAACTCAGAGGTG(+CAGTGT)GTACCTGGTTTCCCATGTGGGTCTAAGCAGT NGG 10 8 17 8 24 0 100 5 10
    240605134311_ptws CCAAGACTGCTTAGACCCACATGGGAAACCAG(+CAGTGT)GTACCACCTCTGAGTTTTCTCTATACAGCTG NGG 10 8 17 8 24 0 100 5 10