Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134242_cxeu GTCAGGAGAACACTTGAAAGGCTTTCTTTGTG(+CAGTGT)GTACCTACGGGGTGAAATCACAGCCTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240605134240_4cgw CTCTCTTGACAGGCTGTGATTTCACCCCGTAG(+CAGTGT)GTACCACAAAGAAAGCCTTTCAAGTGTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605134238_1cot ATTTGAAATGGTTGATAAAATCCTCAAGCTAG(+CAGTGT)GATCCCCCAGAAGGTGTTTGATTGCTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605134236_r7ql AAGGGAACAAAGCAATCAAACACCTTCTGGGG(+CAGTGT)GATCCTAGCTTGAGGATTTTATCAACCATTT NGG 10 8 17 8 24 0 100 5 10
    240605134235_pyzy ATCATCTTACCATTCTGATCCTTCATGCTCTG(+CAGTGT)GTACCCGAGCTCGGAGCGCCCCATCCGTCTA NGG 10 8 17 8 24 0 100 5 10
    240605134233_11n5 ACAGGTAGACGGATGGGGCGCTCCGAGCTCGG(+CAGTGT)GTACCAGAGCATGAAGGATCAGAATGGTAAG NGG 10 8 17 8 24 0 100 5 10
    240605134232_z9wi AACATAATTAGAAAGTCTCCTTCTGGCTGATG(+CAGTGT)GTACCACTGGTCTCTCACCACACTAAGGTTT NGG 10 8 17 8 24 0 100 5 10
    240605134230_php7 TGGCAAAACCTTAGTGTGGTGAGAGACCAGTG(+CAGTGT)GTACCATCAGCCAGAAGGAGACTTTCTAATT NGG 10 8 17 8 24 0 100 5 10
    240605134228_481t GCTAGTCCTATACTTTTACTTAATCTTCTCTG(+CAGTGT)GTACCAGGTAATATTCTCTTTCTTTCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605134226_wcga CTGGGCAGGAGAAAGAAAGAGAATATTACCTG(+CAGTGT)GTACCAGAGAAGATTAAGTAAAAGTATAGGA NGG 10 8 17 8 24 0 100 5 10