Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134339_5h2v AATCAGCTTGCTTTGTTGGCCTAGATATTTTG(+CAGTGT)GATCCTTTATTGAAATGAGGCAGTGAGATAG NGG 10 8 17 8 24 0 100 5 10
    240605134338_348c GGAAACTATCTCACTGCCTCATTTCAATAAAG(+CAGTGT)GATCCAAAATATCTAGGCCAACAAAGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240605134336_jgxt AGTGGAAATAATTTGCTGTCGTTTCAGACTTG(+CAGTGT)GATCCTACAAGCCTGTCCGTTCTGTAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605134335_uatr CAGTACCACTTACAGAACGGACAGGCTTGTAG(+CAGTGT)GATCCAAGTCTGAAACGACAGCAAATTATTT NGG 10 8 17 8 24 0 100 5 10
    240605134334_m8th TGACAAGGGAGTCGGAAGCTAAGGAGGTGACG(+CAGTGT)GATCCTAATGCTTGTCAAATAAACCGATCGC NGG 10 8 17 8 24 0 100 5 10
    240605134333_zgtf TTTTGGCGATCGGTTTATTTGACAAGCATTAG(+CAGTGT)GATCCGTCACCTCCTTAGCTTCCGACTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605134331_aq6v TATCCGTGCTGGAATGGAATGTTAACTGACAG(+CAGTGT)GATCCTGTGTGTGTGTGTGTGTGTGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605134330_vksl ACACACACACACACACACACACACACACACAG(+CAGTGT)GATCCTGTCAGTTAACATTCCATTCCAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605134328_jek7 TATGCGATGGTCTAATAATTAAACGATACTGG(+CAGTGT)GTACCCAATGAGTTTTCAGAGACTGCTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605134327_nw8x CTTGTACACAGCAGTCTCTGAAAACTCATTGG(+CAGTGT)GTACCCAGTATCGTTTAATTATTAGACCATC NGG 10 8 17 8 24 0 100 5 10