Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134141_zizv CAAGGTTTGAGGATATTTGAATAAAATTTTTG(+CAGTGT)GATCCAGCTGCAACATAAAGAGTGCCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605134140_mmk7 ATGTGGGAAGGGCACTCTTTATGTTGCAGCTG(+CAGTGT)GATCCAAAAATTTTATTCAAATATCCTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605134138_ftgg TGAAGCAATAGAAACATGCGATTTAGCTTTGG(+CAGTGT)GATCCTCTGAATGAGGGATTCCCCCAAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605134137_ldpt TTATAGCCTTTGGGGGAATCCCTCATTCAGAG(+CAGTGT)GATCCCAAAGCTAAATCGCATGTTTCTATTG NGG 10 8 17 8 24 0 100 5 10
    240605134135_6mvi AGCCCCCTCCCCCATCGAATCATCCCCCCAGG(+CAGTGT)GTACCCCAGCTAGTCACATACAAAACAGTTT NGG 10 8 17 8 24 0 100 5 10
    240605134133_vcj7 AGACAAAACTGTTTTGTATGTGACTAGCTGGG(+CAGTGT)GTACCCTGGGGGGATGATTCGATGGGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605134131_79tq ACAAAGACAAGGACCAGAAGCAAATACTGGTG(+CAGTGT)GATCCAGATGTTTGAAATCTTTGTATCAAAA NGG 10 8 17 8 24 0 100 5 10
    240605134130_2g5u ACACGTTTTGATACAAAGATTTCAAACATCTG(+CAGTGT)GATCCACCAGTATTTGCTTCTGGTCCTTGTC NGG 10 8 17 8 24 0 100 5 10
    240605134128_kg2z GCTCAACAATACAGAGAATATGTGAACCTGAG(+CAGTGT)GTACCCTGACTCAAATTTCACAACCACAATG NGG 10 8 17 8 24 0 100 5 10
    240605134126_ripz GGTTTCATTGTGGTTGTGAAATTTGAGTCAGG(+CAGTGT)GTACCTCAGGTTCACATATTCTCTGTATTGT NGG 10 8 17 8 24 0 100 5 10