Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134709_qpky AGTAGATGCTCTTGGCTAATGGGTAAGGCGTG(+CAGTGT)GTACCAGAAGCCTATGGCTTTTAGTTATGGT NGG 10 8 17 8 24 0 100 5 10
    240605134708_6b3s CCACAACCATAACTAAAAGCCATAGGCTTCTG(+CAGTGT)GTACCACGCCTTACCCATTAGCCAAGAGCAT NGG 10 8 17 8 24 0 100 5 10
    240605134707_85ud GGCGTCCTGGGATGGCACAGCCTCCCTCTGAG(+CAGTGT)GTACCTAGGGAGATGTTCTAAGACCTGGGCG NGG 10 8 17 8 24 0 100 5 10
    240605134704_14aq CTGTGCGCCCAGGTCTTAGAACATCTCCCTAG(+CAGTGT)GTACCTCAGAGGGAGGCTGTGCCATCCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605134703_hipy CCCAGCTCCAGTTGGCTACACGGCTACACGCG(+CAGTGT)GATCCCTGCTCTGGCCCACTCACCCTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605134701_1d7y GTAGGCAGGCAGGGTGAGTGGGCCAGAGCAGG(+CAGTGT)GATCCGCGTGTAGCCGTGTAGCCAACTGGAG NGG 10 8 17 8 24 0 100 5 10
    240605134700_dqib GCCAGTCATCTGATGTGTCAAAAGTCTTCAAG(+CAGTGT)GATCCTTTGCTAGAGCTTGTCTGCAGATGGA NGG 10 8 17 8 24 0 100 5 10
    240605134658_ap09 TCAGCTCCATCTGCAGACAAGCTCTAGCAAAG(+CAGTGT)GATCCTTGAAGACTTTTGACACATCAGATGA NGG 10 8 17 8 24 0 100 5 10
    240605134657_j4fd GACAGTCATCTGATGTGTCAAAAGTCTTCAAG(+CAGTGT)GATCCTTTGCCAGAGCTTGTCTGCAGATGGA NGG 10 8 17 8 24 0 100 5 10
    240605134656_7zpa TCAGGTCCATCTGCAGACAAGCTCTGGCAAAG(+CAGTGT)GATCCTTGAAGACTTTTGACACATCAGATGA NGG 10 8 17 8 24 0 100 5 10