Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134035_f1ag GAGTGCTGATAAAGGCATCTGGGGGCCCAGAG(+CAGTGT)GATCCCAGTAGAGACTGCTTGCTCTATGTAT NGG 10 8 17 8 24 0 100 5 10
    240605134033_7434 CTGTAATACATAGAGCAAGCAGTCTCTACTGG(+CAGTGT)GATCCTCTGGGCCCCCAGATGCCTTTATCAG NGG 10 8 17 8 24 0 100 5 10
    240605134032_uryd GTTACTGTTCATTTCTGGAGTCTGATGTTCTG(+CAGTGT)GTACCCAGCTCTGGTGTCCCTGCCAGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605134030_1476 TTTCTCAGCCCTGGCAGGGACACCAGAGCTGG(+CAGTGT)GTACCAGAACATCAGACTCCAGAAATGAACA NGG 10 8 17 8 24 0 100 5 10
    240605134029_3r3z GACACACTCCCTTGAGTTCTTGGGCAACTTGG(+CAGTGT)GTACCTGATGAGTGGCCTTTGAGCTTAACTG NGG 10 8 17 8 24 0 100 5 10
    240605134027_3fr8 TGTTTCAGTTAAGCTCAAAGGCCACTCATCAG(+CAGTGT)GTACCCAAGTTGCCCAAGAACTCAAGGGAGT NGG 10 8 17 8 24 0 100 5 10
    240605134025_asvu ATGTGGTGCCTGTTCAAAGTCCTGCATAAGGG(+CAGTGT)GATCCCTGTACACTCGAACCGTTATTATTTG NGG 10 8 17 8 24 0 100 5 10
    240605134024_qb6s ATCATCAAATAATAACGGTTCGAGTGTACAGG(+CAGTGT)GATCCCCTTATGCAGGACTTTGAACAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240605134023_vxgc CTGTGTGTAGCATTGAATGGCTTCAGGGTTTG(+CAGTGT)GATCCCGTTGAGTGGGGCAAGGTAGGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240605134021_r05d ACTTGGGCCTCCTACCTTGCCCCACTCAACGG(+CAGTGT)GATCCAAACCCTGAAGCCATTCAATGCTACA NGG 10 8 17 8 24 0 100 5 10