Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134002_kq1k AAACAAACAATCTATGATAACTCTGTACAGAG(+CAGTGT)GTACCAAACCTTTAAGTATTTGTGATGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605134001_cpmc TCAAATTCCCATCACAAATACTTAAAGGTTTG(+CAGTGT)GTACCTCTGTACAGAGTTATCATAGATTGTT NGG 10 8 17 8 24 0 100 5 10
    240605133959_uup2 TAATTCAGGGGCTACAGCTCCAAGCCAAGCAG(+CAGTGT)GTACCAGGGCACGATGGATCGAAAGGCAATA NGG 10 8 17 8 24 0 100 5 10
    240605133957_lk7v CCACATATTGCCTTTCGATCCATCGTGCCCTG(+CAGTGT)GTACCTGCTTGGCTTGGAGCTGTAGCCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605133955_zoky GGCATGGCCTTTCCTCAGAACTTTCTACCATG(+CAGTGT)GATCCAAAGCCTAAGACTAGGCCATCGTAGA NGG 10 8 17 8 24 0 100 5 10
    240605133953_1fda GATGGTCTACGATGGCCTAGTCTTAGGCTTTG(+CAGTGT)GATCCATGGTAGAAAGTTCTGAGGAAAGGCC NGG 10 8 17 8 24 0 100 5 10
    240605133952_len2 GGGCAGACAAGGCAGCCCAGTTAGGGAGACAG(+CAGTGT)GATCCACAGATAGGCAACGCATTTAAGGACA NGG 10 8 17 8 24 0 100 5 10
    240605133951_f37f GGGGCTGTCCTTAAATGCGTTGCCTATCTGTG(+CAGTGT)GATCCTGTCTCCCTAACTGGGCTGCCTTGTC NGG 10 8 17 8 24 0 100 5 10
    240605133949_q0e2 GGATGGAGCCTCTCTGAGGACAGCTATACTAG(+CAGTGT)GATCCTGTCTGCAAGCATAACAGAGTATCAT NGG 10 8 17 8 24 0 100 5 10
    240605133948_vppx TATTAATGATACTCTGTTATGCTTGCAGACAG(+CAGTGT)GATCCTAGTATAGCTGTCCTCAGAGAGGCTC NGG 10 8 17 8 24 0 100 5 10