Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605133857_pu0f AAATCACTGCCCCATACTCAAACGACTCCCAG(+CAGTGT)GTACCCATTACTAGAAGTTTCTAAGTCACAG NGG 10 8 17 8 24 0 100 5 10
    240605133855_xk1r GGAGCCTGTGACTTAGAAACTTCTAGTAATGG(+CAGTGT)GTACCTGGGAGTCGTTTGAGTATGGGGCAGT NGG 10 8 17 8 24 0 100 5 10
    240605133854_dyra AAGCCACTTAAAGACCTATACACAACGTGATG(+CAGTGT)GTACCTTAGAATTCTGTAGTTGGGGAATTTC NGG 10 8 17 8 24 0 100 5 10
    240605133853_8js7 AATGTGAAATTCCCCAACTACAGAATTCTAAG(+CAGTGT)GTACCATCACGTTGTGTATAGGTCTTTAAGT NGG 10 8 17 8 24 0 100 5 10
    240605133851_sgla CTGTCTCTGCATCAGCTGACCATCTTCCAGTG(+CAGTGT)GTACCTCTCCTCCCCTGCTCACCCCTCACCA NGG 10 8 17 8 24 0 100 5 10
    240605133850_hrtr CCATCTGGTGAGGGGTGAGCAGGGGAGGAGAG(+CAGTGT)GTACCACTGGAAGATGGTCAGCTGATGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605133848_kv97 CAACTCTCTAGGCCTTACTTTGGGGAGCCTGG(+CAGTGT)GATCCTCTTCCAATTTCTCCATTTGGCCCCA NGG 10 8 17 8 24 0 100 5 10
    240605133846_k5qg AGAGTTGGGGCCAAATGGAGAAATTGGAAGAG(+CAGTGT)GATCCCAGGCTCCCCAAAGTAAGGCCTAGAG NGG 10 8 17 8 24 0 100 5 10
    240605133845_apsj ACCACCCTGGAATAGGGGCATCAAGATAGGAG(+CAGTGT)GTACCCAGTTCAGAAGGTGAGTCCCAAATAC NGG 10 8 17 8 24 0 100 5 10
    240605133843_zycj AGGGGGTATTTGGGACTCACCTTCTGAACTGG(+CAGTGT)GTACCTCCTATCTTGATGCCCCTATTCCAGG NGG 10 8 17 8 24 0 100 5 10