Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605133946_ks1j TTACACGAGTCAGTTCTTTTCTCTCCACCATG(+CAGTGT)GATCCCACTGATCAAACCTGGGTTATCAGCC NGG 10 8 17 8 24 0 100 5 10
    240605133944_54o1 TGCAAGGCTGATAACCCAGGTTTGATCAGTGG(+CAGTGT)GATCCATGGTGGAGAGAAAAGAACTGACTCG NGG 10 8 17 8 24 0 100 5 10
    240605133942_x8uj TTTAAGCCCACCTGGTCACCCATCAAAGTCCG(+CAGTGT)GATCCTGTCTTTCCCCGTTTACCCTTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240605133941_aqub GGAGGGTGGGAAGGGTAAACGGGGAAAGACAG(+CAGTGT)GATCCGGACTTTGATGGGTGACCAGGTGGGC NGG 10 8 17 8 24 0 100 5 10
    240605133939_33ie GCTTTATTCATTATTGACATTTGTAGTTGAGG(+CAGTGT)GATCCGGGGGTTCAATTTATTTTGATTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605133936_as4j AAAAAAAAAAATCAAAATAAATTGAACCCCCG(+CAGTGT)GATCCCTCAACTACAAATGTCAATAATGAAT NGG 10 8 17 8 24 0 100 5 10
    240605133935_0b7z TGGCCACCACCTCCCCCGGACTCTACCTCAAG(+CAGTGT)GATCCACCTCAAACCAAGCCTGACCCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605133933_ci6f GGTGATGCCTGGGTCAGGCTTGGTTTGAGGTG(+CAGTGT)GATCCTTGAGGTAGAGTCCGGGGGAGGTGGT NGG 10 8 17 8 24 0 100 5 10
    240605133931_ooum AAGGAGGCGACGCAGAGGCACTGGGGTACAGG(+CAGTGT)GTACCAAATGAAAGAGAGAGAGTCTTGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605133930_obgq CTCCATTCTCAAGACTCTCTCTCTTTCATTTG(+CAGTGT)GTACCCTGTACCCCAGTGCCTCTGCGTCGCC NGG 10 8 17 8 24 0 100 5 10