Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605133842_vt2p GGTCACTGCAGGGGAATGGTCTCAAGCTGCAG(+CAGTGT)GATCCTAACCTGGAAGAGGAAGCCATGGTCT NGG 10 8 17 8 24 0 100 5 10
    240605133840_j5ih TGAACAGACCATGGCTTCCTCTTCCAGGTTAG(+CAGTGT)GATCCTGCAGCTTGAGACCATTCCCCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605133839_paeh ATCGATCCAGACGAGGTCAGGGCTGTGCTGTG(+CAGTGT)GATCCATCACACAAGCGTCTGTCCATCCCAT NGG 10 8 17 8 24 0 100 5 10
    240605133838_cfjy GAGAGATGGGATGGACAGACGCTTGTGTGATG(+CAGTGT)GATCCACAGCACAGCCCTGACCTCGTCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605133836_2u59 AAGCCTCTGCACTCTTGCCATCCCGTGTCAGG(+CAGTGT)GATCCAGTGTGGGTGGGCGGCTGAGGACCTC NGG 10 8 17 8 24 0 100 5 10
    240605133835_fdti GGAAGGAGGTCCTCAGCCGCCCACCCACACTG(+CAGTGT)GATCCCTGACACGGGATGGCAAGAGTGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605133833_q2ic AAAACAGCAGCCACCTATGGACCAGTCCATTG(+CAGTGT)GTACCATACCAATCTGGAAAGGGCACGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605133832_hpg8 CCCAGCTGGCGTGCCCTTTCCAGATTGGTATG(+CAGTGT)GTACCAATGGACTGGTCCATAGGTGGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605133831_ar91 GGCGCAGGAGGGGCCTGCCCCCCCACACACTG(+CAGTGT)GATCCCCAGTGGCCAAGTGCCCCGGGCCTTT NGG 10 8 17 8 24 0 100 5 10
    240605133829_wbya ATGTGAAAGGCCCGGGGCACTTGGCCACTGGG(+CAGTGT)GATCCAGTGTGTGGGGGGGCAGGCCCCTCCT NGG 10 8 17 8 24 0 100 5 10