Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605133827_ox7a CCTAGCCTCTGAGCATAGAGGCTGAGGGCCTG(+CAGTGT)GTACCTGAGTTTTAAAAACAAGTTAATTACT NGG 10 8 17 8 24 0 100 5 10
    240605133826_6pxo TACAAAGTAATTAACTTGTTTTTAAAACTCAG(+CAGTGT)GTACCAGGCCCTCAGCCTCTATGCTCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605133824_8can ACACTCAGAAGTTGCATTCAACAGGGCCACTG(+CAGTGT)GATCCCCTTGGGATTCGGTGCCAGATATTAC NGG 10 8 17 8 24 0 100 5 10
    240605133822_47g0 ACCCTGTAATATCTGGCACCGAATCCCAAGGG(+CAGTGT)GATCCAGTGGCCCTGTTGAATGCAACTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605133820_cy9s GGTGCACATCCTATGCCGCACAGCGGTGAGTG(+CAGTGT)GATCCCGGCCAAGAGAATACTCCAGTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605133818_qccs GATGTCTGAGACTGGAGTATTCTCTTGGCCGG(+CAGTGT)GATCCACTCACCGCTGTGCGGCATAGGATGT NGG 10 8 17 8 24 0 100 5 10
    240605133817_r306 TTAGGCAAAGTTAATAGGGTATCTGGCATTGG(+CAGTGT)GATCCTACTGACTGCCATTCAGTAAGCCAAT NGG 10 8 17 8 24 0 100 5 10
    240605133816_dx5g TAACTATTGGCTTACTGAATGGCAGTCAGTAG(+CAGTGT)GATCCCAATGCCAGATACCCTATTAACTTTG NGG 10 8 17 8 24 0 100 5 10
    240605133813_rv0k TTCAGGGAAAAAGAAAACATGTTTTACCTAGG(+CAGTGT)GATCCTTCTGAACAAAATGGCTGTCTGTACT NGG 10 8 17 8 24 0 100 5 10
    240605133812_h6dm AAAAGAGTACAGACAGCCATTTTGTTCAGAAG(+CAGTGT)GATCCCTAGGTAAAACATGTTTTCTTTTTCC NGG 10 8 17 8 24 0 100 5 10