Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134051_icyj CCAGCAGGGAGTGGAGGACTCGGACTCTGGGG(+CAGTGT)GATCCTACTGCTTGCATAGGTCCTGGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605134050_su58 CTAAGCCACACCAGGACCTATGCAAGCAGTAG(+CAGTGT)GATCCCCCAGAGTCCGAGTCCTCCACTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605134049_o97j TCTGCCTCGAACTAATTCATCTCACGCTGTTG(+CAGTGT)GTACCGAGTGTGAGGCTCAGCGACTGGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605134047_5ug1 TGATTTTCTCCAGTCGCTGAGCCTCACACTCG(+CAGTGT)GTACCAACAGCGTGAGATGAATTAGTTCGAG NGG 10 8 17 8 24 0 100 5 10
    240605134046_vbf1 TCCCTTGCCTAAGGCAGAGCCGTTTCCTTCAG(+CAGTGT)GTACCACACAAGCAATTTGCAGACCTGACTC NGG 10 8 17 8 24 0 100 5 10
    240605134045_z78c TTTCTGAGTCAGGTCTGCAAATTGCTTGTGTG(+CAGTGT)GTACCTGAAGGAAACGGCTCTGCCTTAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605134043_hfpi ATAGAAAAGGGTGGTATCTGGGAGTCTAAGGG(+CAGTGT)GTACCTTTTCATCTGACCTACTGACCAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605134041_6qaz CTATGACTTTGGTCAGTAGGTCAGATGAAAAG(+CAGTGT)GTACCCCTTAGACTCCCAGATACCACCCTTT NGG 10 8 17 8 24 0 100 5 10
    240605134039_8ixt TCCATGCTACCTACCAATAAAACCAGGCCCTG(+CAGTGT)GTACCAAAGGCCTGTGGAGTACTGGGCACTG NGG 10 8 17 8 24 0 100 5 10
    240605134037_b9tp CAGCGCAGTGCCCAGTACTCCACAGGCCTTTG(+CAGTGT)GTACCAGGGCCTGGTTTTATTGGTAGGTAGC NGG 10 8 17 8 24 0 100 5 10