Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134018_xxcx CAGCCAGGCTGGGGGTACATGTAGCACCCCTG(+CAGTGT)GATCCCATCTTGTTGCTAGCAGGGTAAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605134016_k10r AGGGGTCACTTACCCTGCTAGCAACAAGATGG(+CAGTGT)GATCCAGGGGTGCTACATGTACCCCCAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605134015_6yle GCCTGCTGTTCATCTGACAAGCAAGAGCAGTG(+CAGTGT)GATCCATTTCTAAGGCTGTGTACAGGAAGAC NGG 10 8 17 8 24 0 100 5 10
    240605134014_68fz CCACTGTCTTCCTGTACACAGCCTTAGAAATG(+CAGTGT)GATCCACTGCTCTTGCTTGTCAGATGAACAG NGG 10 8 17 8 24 0 100 5 10
    240605134012_h0mk AGTCCTCCCCTGACTCTACCTAGCTAATCCTG(+CAGTGT)GATCCCATTTTACTTAGGAAAACAAAAACAA NGG 10 8 17 8 24 0 100 5 10
    240605134010_73at TAAGTTTGTTTTTGTTTTCCTAAGTAAAATGG(+CAGTGT)GATCCAGGATTAGCTAGGTAGAGTCAGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605134009_t3oi AAGTGGATTTCCGAGATGAGGGTTGGCACTTG(+CAGTGT)GTACCCACCTGTCGAGTTCTGTGGGACGTCA NGG 10 8 17 8 24 0 100 5 10
    240605134007_eaf1 GAGCATGACGTCCCACAGAACTCGACAGGTGG(+CAGTGT)GTACCAAGTGCCAACCCTCATCTCGGAAATC NGG 10 8 17 8 24 0 100 5 10
    240605134005_vwwm GCTCTGCATTACAAATGTCCTAATGGTGCCAG(+CAGTGT)GTACCTTCTGAAGACTCACAGGAGACATTAC NGG 10 8 17 8 24 0 100 5 10
    240605134004_n10g CTTGAGTAATGTCTCCTGTGAGTCTTCAGAAG(+CAGTGT)GTACCTGGCACCATTAGGACATTTGTAATGC NGG 10 8 17 8 24 0 100 5 10