Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134657_j4fd GACAGTCATCTGATGTGTCAAAAGTCTTCAAG(+CAGTGT)GATCCTTTGCCAGAGCTTGTCTGCAGATGGA NGG 10 8 17 8 24 0 100 5 10
    240605134656_7zpa TCAGGTCCATCTGCAGACAAGCTCTGGCAAAG(+CAGTGT)GATCCTTGAAGACTTTTGACACATCAGATGA NGG 10 8 17 8 24 0 100 5 10
    240605134655_u6ux GCCAGTCATCTGATATGTCAAAAGTATTCAAG(+CAGTGT)GATCCTTTTCCAGAGCTTGTCTGCAGATGGA NGG 10 8 17 8 24 0 100 5 10
    240605134653_ympw TCAGGTCCATCTGCAGACAAGCTCTGGAAAAG(+CAGTGT)GATCCTTGAATACTTTTGACATATCAGATGA NGG 10 8 17 8 24 0 100 5 10
    240605134651_abrs AACTGAGTAGCAGGAGGAAAAGGAGGGCCAGG(+CAGTGT)GATCCTGGTGTAGTGAGCAGGGTGGTTAGCT NGG 10 8 17 8 24 0 100 5 10
    240605134649_p1zr TGTTAAGCTAACCACCCTGCTCACTACACCAG(+CAGTGT)GATCCCTGGCCCTCCTTTTCCTCCTGCTACT NGG 10 8 17 8 24 0 100 5 10
    240605134647_78u0 TAGCCTTCACCCAGAAGCATGCATGGCCTGAG(+CAGTGT)GTACCCATGGGTTATCTAACTTCTCAGAATC NGG 10 8 17 8 24 0 100 5 10
    240605134645_5tdv GAGTGGATTCTGAGAAGTTAGATAACCCATGG(+CAGTGT)GTACCTCAGGCCATGCATGCTTCTGGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605134644_e8v4 GCAATCTTCTTGCAATCCCTGACCTATTTGGG(+CAGTGT)GTACCTTAGAACCTCCTCTTGGCTAGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605134642_zwon AGCATCCTCTCTAGCCAAGAGGAGGTTCTAAG(+CAGTGT)GTACCCCAAATAGGTCAGGGATTGCAAGAAG NGG 10 8 17 8 24 0 100 5 10