Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605134802_u1w9 ATCAGCATGCCTGCCTCATGGGATTAAAGGTG(+CAGTGT)GTACCACCATGCCTGGATCTAATTAAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605134800_aqsb TCTACCCAGCTTAATTAGATCCAGGCATGGTG(+CAGTGT)GTACCACCTTTAATCCCATGAGGCAGGCATG NGG 10 8 17 8 24 0 100 5 10
    240605134758_uhl9 GTCAGGCCATTCACAGCAATATACCACTCCTG(+CAGTGT)GTACCAACTTCTGTCTTAGTTAGGGTTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605134757_j264 CAGCAGTAAAACCCTAACTAAGACAGAAGTTG(+CAGTGT)GTACCAGGAGTGGTATATTGCTGTGAATGGC NGG 10 8 17 8 24 0 100 5 10
    240605134756_fq7e CATTAATTACTAAAGCTCTTTATATAGGAGAG(+CAGTGT)GATCCTCTTGAGATTTGTCCATCAATGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605134755_gfhn ACATGCCAGCATTGATGGACAAATCTCAAGAG(+CAGTGT)GATCCTCTCCTATATAAAGAGCTTTAGTAAT NGG 10 8 17 8 24 0 100 5 10
    240605134753_u4q9 ATTATGTAAACTCTAGACCCCGAAACTTCCAG(+CAGTGT)GATCCTCTGTTCTCAGATGCTCTGGTGATCT NGG 10 8 17 8 24 0 100 5 10
    240605134752_zs27 TAGGCAGATCACCAGAGCATCTGAGAACAGAG(+CAGTGT)GATCCTGGAAGTTTCGGGGTCTAGAGTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605134750_dm8j TCACTCAGGATATTGTTACAGGACATGCAGGG(+CAGTGT)GATCCAATCCCTTCCACACCTTCCGACCCTT NGG 10 8 17 8 24 0 100 5 10
    240605134749_fl1j AGGAGAAGGGTCGGAAGGTGTGGAAGGGATTG(+CAGTGT)GATCCCCTGCATGTCCTGTAACAATATCCTG NGG 10 8 17 8 24 0 100 5 10